...exhibitor booth in order to help scientists find the plasmids they need for their research, show labs how they...about all the amazing research being done with plasmids. I heard an impressive talk about the use of optogenetics...and resources by storing and distributing their plasmids with the scientific community. All of this speaks...

...distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids. Our most exciting...

...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...

...put to such great use. It’s just one of the many plasmids being distributed to and accelerating research...Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website...Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...

...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with... in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization ...

...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...

...is interesting because by changing certain amino acids you can change the specificity,” explained Zhanar...consecutive base in its RNA target. By changing amino acids, it’s possible to produce a protein that binds an...and improve, Abil says they will make those new plasmids available through Addgene, and they hope others...

...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

....1420024112. Epub 2015 Feb 23. Pubmed. Find the plasmids from this publication at Addgene. Dynamic imaging...10.1016/j.cell.2013.12.001. Pubmed. Find the plasmids from this publication at Addgene. A protein-....2014.09.039. Epub 2014 Oct 9. Pubmed. Find the plasmids from this publication at Addgene. ...

....1111704108. Epub 2011 Aug 9. PubMed. Find the Plasmids @Addgene: Plasmids for KD, R, B2, and B3 recombinases from... LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/gkl1108...

...thanks for depositing your plasmids with Addgene. Howarth: We submit all our plasmids that may well be of use...encoded way. SpyTag is nice and small, just 13 amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher...

...Addgene.org Find plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent...acoustic reporters for your research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau...

...tools (and can have secondary functions as detection aids.) When a single protein is expressed, with the intent...antibodies, resins, beads, and other purification aids readily available. Choosing the best tag for your...Antibody protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags...

...steps into this field, we’ve curated microbiology plasmids from the repository onto one handy Microbiology...these new Rainbow Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday...Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

...genome editing using the Zhang lab's CRISPR/Cas plasmids. Designing your CRISPR genome editing experiment...related to the most frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools...by a variety of labs and optimized for specific plasmids Links to different software to help you identify...

... in the field, and enabling access to multiple plasmids used for various platform applications. For more... out Addgene’s CRISPR Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...Resources on the Addgene Blog Check out other Hot Plasmids Find Additional Posts on Mol Bio Techniques Find... Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

...protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids ...translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by the ribosome. ...

...transduce? So why use a virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The...typically transfected along with viral packaging plasmids into a viral production cell line, usually HEK...HEK-293 cells. When all of these viral plasmids are expressed within the production cell line, viral particles...

...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...