We narrowed to 782 results for: ina
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TypeBlog PostPublishedMay 12, 2022, 1:15 p.m....The weather is finally warming up in Boston and that means summer is right around the corner. Here at...other video series. And if you haven’t seen our webinar “Navigating an Expanding Landscape for Engineered...which we will be posting in the next few weeks.) Finally, Addgenies love sunshine and warm weather - check...
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New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m....of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs Kit...Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's Lab -... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog PostPublishedApril 5, 2016, 2:30 p.m....taking steps to increase plasmid distribution and disseminate knowledge around the globe. Addgene’s progress...inception in 2004. In the near future, we hope to eliminate much of white space on this map and increase plasmid...the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....project that would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD ... Tsang, Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting...customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... work with in neurons or you might use it in combination with Flp-FRT. Additionally, you could use the... cre-recombinase... -
Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m....lab heads have revealed that a combination of hard work, determination, passion, and patience are required... time for fun and life outside of the lab? This final post in the PI Interview Series investigates how... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....distributor partners, with the launch of antibodies in China and expansion of viral services available in South...Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first...partially through work with our distributors in China, Korea, Japan, India, and Brazil. Through an expansion... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....the process a bit easier and more efficient. Our original policy was straightforward, but cumbersome: depositors...champagne or cupcakes, or maybe the lab member originally responsible for the deposit had already moved...And we can update your plasmids’ citation to the final journal article version any time later. We recognize... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m.... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m....as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase cleaves only...and images in this post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome...For more information and a link to the archived webinar, click here. We would like to thank IDT scientists... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....significant improvement and simplification of the original technique, specifically in terms of initial steps...excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there... a graduate student at the University of North Carolina in Chapel Hill, where he studies enhancers and... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog PostPublishedApril 25, 2024, 1:15 p.m....this goal could involve participating in interdisciplinary seminars, such as those in bioinformatics or chemistry...lies within a biological lab. Engaging in such seminars exposes you to discussions beyond your immediate... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....physical association of combinations of different Gα, Gβ, and Gγ subunits. When inactive, these subunits are...addition of the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....’s functions, besides structure and isotype determination, don’t generally affect antibody applications...stabbing” and “poisoning,” but let’s be honest, the elimination style the immune system is most famous for is...domain as only providing structure and isotype determination, remember that it can do so much more than that... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog PostPublishedOct. 18, 2017, 1 p.m....then in my final year of graduate school, I actually served as the student course coordinator. With the...’d put in outside the lab had paid off. “The combination of the editing and teaching experience that I... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....perfectionists, it took us a full two months to finally decide the LEGO laboratory was good enough. There...box, the Big Bang Theory set, and then use our imaginations to mold it all into a full laboratory. It is...It was a great treat for us to put our rusty imaginations to use in this world where we are often trained... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....the advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses ... are many such sites in the human genome). The original plasmid is empty following the removal of the ...transposon sequences and then testing various combinations of changes. The basic functional version of ... -
10 Ways to Share Your Science!
TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....North Carolina Research Triangle area, scientists give talks and science cafés at the North Carolina Museum... surprised to find that his friends and family finally understood why he cared to study mice on some distant... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... precious in less resourced areas of the world. Nina Dudnik, Founder of Seeding Labs, tells the story...Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent...this was hilarious. Birth of the baby gel box Finally, taking conservation to new levels, I was bothered... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....kill bacteria, or inhibit their growth. Although originally sourced from natural products, many common antibiotics... are trying to disrupt or delete, which both inactivates the gene and acts as a marker for the mutation...cassettes and your gene of interest is cloned into/inactivates or completely removes (in the case of Gateway...