...as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase cleaves only...and images in this post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome...For more information and a link to the archived webinar, click here. We would like to thank IDT scientists...

...significant improvement and simplification of the original technique, specifically in terms of initial steps...excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there... a graduate student at the University of North Carolina in Chapel Hill, where he studies enhancers and...

...physical association of combinations of different Gα, Gβ, and Gγ subunits. When inactive, these subunits are...addition of the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8...

...’s functions, besides structure and isotype determination, don’t generally affect antibody applications...stabbing” and “poisoning,” but let’s be honest, the elimination style the immune system is most famous for is...domain as only providing structure and isotype determination, remember that it can do so much more than that...

...this goal could involve participating in interdisciplinary seminars, such as those in bioinformatics or chemistry...lies within a biological lab. Engaging in such seminars exposes you to discussions beyond your immediate...

...then in my final year of graduate school, I actually served as the student course coordinator. With the...’d put in outside the lab had paid off. “The combination of the editing and teaching experience that I...

...the advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses ... are many such sites in the human genome). The original plasmid is empty following the removal of the ...transposon sequences and then testing various combinations of changes. The basic functional version of ...

...North Carolina Research Triangle area, scientists give talks and science cafés at the North Carolina Museum... surprised to find that his friends and family finally understood why he cared to study mice on some distant...

... precious in less resourced areas of the world.  Nina Dudnik, Founder of Seeding Labs, tells the story...Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent...this was hilarious.  Birth of the baby gel box Finally, taking conservation to new levels, I was bothered...

...forHomologous Recombination (HR)? How long should the homology arms be for efficient recombination? A3: The... the CRISPR/Cas system with your homologous recombination (HR) template, you could then: Confirm your...

...kill bacteria, or inhibit their growth. Although originally sourced from natural products, many common antibiotics... are trying to disrupt or delete, which both inactivates the gene and acts as a marker for the mutation...cassettes and your gene of interest is cloned into/inactivates or completely removes (in the case of Gateway...

...unsuccessful. Nothing more joyful that getting to the final step and the transformed bacteria refuse to grow...(Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). Both rabbit (EMD, AB3596) and mouse (NEB, E8032S...matrices, not so much. Maybe a calcium-independent proteinase site could be used for plasmid version 2.0? Instruments...

...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

...Di Ventura (@barbara_synbio) May 13, 2021 The original AraC transcriptional regulator Their system is...does BLADE have over AraC? When compared to the original AraC system, BLADE is simply more precise. “We...light switch and shuts off just as quickly. The original AraC system, which uses arabinose, requires many...

...mobilized to figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...

...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...scientists think that they are unable to tick these final two boxes, but I knew there would be a way around...marketing gives you a great introduction to the financial world. It teaches you about selling products which...

...This is the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series...SMART.” SMART goals are Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea ... open communication Feel valued by giving back Final tips for success Make mentoring meetings a priority...

... a means of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed... 7. S.-L. Lai, T. Lee, Genetic mosaic with dual binary transcriptional systems in Drosophila., Nat. Neurosci..., L. Liang, L. Luo, The Q system: a repressible binary system for transgene expression, lineage tracing...

...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...heterogeneous and/or mixed clones present in the original sample. At seqWell, we have worked with Addgene...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

...Cas9 activators available in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate some of...any questions or comments to [email protected]. Finally we’d like to thank all those who are allowing us...