We narrowed to 488 results for: mme

1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...thoughts about this technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex...

2. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ... you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring group ...

3. Will You Be My Mentor? Finding and Asking for Mentoring Support

   Type

   Blog Post

   Published

   Feb. 4, 2014, 3:50 p.m.

   ...and make sure to choose one that comes highly recommended from someone you trust. A good coach can make...

4. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   Published

   Dec. 10, 2013, 3:13 p.m.

   ...the DNA-binding domain is modifiable and can be programmed to bind to essentially any DNA sequence desired...

5. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ... al., 2011).  Additionally, researchers have reprogrammed regenerating tissues without using genome engineering...

6. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are...

7. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...Lucas, Burstein, David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

8. Antibodies 101: Secondary Antibodies

   Type

   Blog Post

   Published

   Dec. 21, 2021, 2:15 p.m.

   ...blot can be run in a single day, I really cannot recommend it except in the most dire of circumstances.) ...

9. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f nuclease...

10. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...protein as the repressor because TALEs can be programmed to bind tightly to arbitrary DNA sequences and...

11. Plasmids 101: The protein expression toolbox

    Type

    Blog Post

    Published

    Feb. 16, 2023, 7:05 p.m.

    ...References References Nabet et al. The dTAG system for immediate and target-specific protein degradation. Nature...

12. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...

13. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...probably seen this study (and various articles and commentaries) pop up on Twitter and Facebook, shared by biologists...

14. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...downstream steps. It’s also possible to determine immediate effects of signaling vs the delayed effects because...

15. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...monomer in the cell type tested. To conclude, we recommend that you try a couple of different fluorescent...

16. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...Tell us your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene...

17. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...hired to produce results, not scientists”  These comments are well correlated with published data. For example...

18. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...Gate assembly is also less expensive than many commercial cloning methods. Golden Gate and synthetic biology...

19. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...searching, reading and organization tips in the comments below. Additionally, read more of the Beginner...

20. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    Published

    Jan. 29, 2015, 1:25 p.m.

    ...consider. Feel free to add other suggestions in the comments or email me with suggestions.  Most coaches have...