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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 679 results for: mos

Showing: 361 - 380 of 679 results

  1. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...it is unpredictable which fluorescent protein is most photostable for each of these conditions. On top...power used for live-cell imaging. To conclude, the most photostable fluorescent protein can be identified...

  2. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...dry lab scientists (or vice versa), then you have almost certainly experienced some of the hurdles I needed...needed to overcome.  I eventually pinpointed and (mostly) solved two main problems with Addgene’s cross ...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...relationships (also known as “networking”), one of the most common questions I receive is “Do I have to be on...to you (unless they’ve chosen to hide it). While most people use LinkedIn messaging, not everyone likes...

  4. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...isolate proteins, this blog will focus on one of the most common, purification based on the polyhistidine ...containing two or more adjacent histidines are present in most production systems and can bind and co-elute with...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes is its method of DNA cleavage...abilities   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas...

  6. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...sharing your science - which can drive away even the most enthusiastic of learners.  What contributes to cognitive...audience to take on, just to understand you at the most basic of levels.  Concepts This can be obvious, ...

  7. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... a number of research and clinical applications. Most of the time, when someone speaks about an affinity...thing to go when downsizing an affinity reagent, most of the alternative reagents have reduced immunogenicity...

  8. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...was successfully used in conditions that are the most similar to yours. Take a look at the publications... You now know that application validation is the most important factor in antibody selection, but what...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ... is further processed to isolate the antibodies. Most commonly, antibodies of the IgG isotype are used...When to stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their...

  10. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...during tissue collection and homogenization. The most widely known of these products is TRIzol® (also ...researchers can do to combat this issue. First and foremost, be mindful of your extractions- if you need really...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ... 145,000 more quality-adjusted life years. While most of the results were positive, the analysis did find...et al. provide a table indicating the sex of the most commonly used cell lines appearing in American Journal...

  13. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...prone to error — both human and biological. Even the most high-fidelity polymerases can make mistakes and ...and highly repetitive sequences and/or features. Most of these regions are identified during the QC process...

  14. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...Primer binding sites with 40–60% G/C content are most likely to be successful, although sequences with...which helps users select pegRNA designs that are most likely to drive efficient editing. This is not the...

  15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    .... As the depositing scientist, you will have the most involvement with Step 1, and, while data entry can...every so often and this ensures you are using our most up-to-date version! We hope the deposit spreadsheet...

  16. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives...

  19. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...similar homology to the site of interest or create mosaic animals where not all cells in the blastocyst were...small molecule that regulates Cre expression. The most common drug used to induce the expression of Cre...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the most photostable yellow fluorescent protein to date By... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...
Showing: 361 - 380 of 679 results