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Showing: 361 - 380 of 650 results

  1. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...course of the last few years: recognizing the importance and benefit of remote and video communication...staff have become a new way to allow you to get important information about the campus even if you can’t...

  2. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...vectors is how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates...

  3. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...biomedical applications of these tools difficult or impossible. But if you’ve ever had an MRI, you know that...the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could be expressed...

  7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...have that conversation out in the open is really important. Addgene: Is there anything else you’d like to...biology is massively underfunded considering how important it is. I would really like to reach all those ...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers developed viral...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  10. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...that can be monitored non-invasively over time. Importantly, it is an immune privileged compartment, meaning...underway and reported results are encouraging. Importantly, a significant amount of basic research is being...

  12. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine... Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector...

  15. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... increasingly politicized. It would be nearly impossible for a career scientist such as Zimmer to introduce...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...

  16. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...toward saying that host-virus interactions are an important part of the biology of adenoviruses (AdVs). AdVs... Although host-virus interactions likely seem important for any virus, we will see that they are very ...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein...In both cases, the lipid membrane is the only component that is not dependent on viral genes. It is derived...

  18. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...will link biomolecules to the gel and add gel components to polymerize. Step 3: digest the sample to allow...isotropic expansion depends on how well the gel components penetrate the sample, how well the gel polymerizes...

  19. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...interaction with other proteins.   It is important to note that research activities in a CURE must...opportunity to make new discoveries. Provost notes the importance of acknowledging student apprehensions about ...

  20. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious...
Showing: 361 - 380 of 650 results