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We narrowed to 662 results for: mpo

Showing: 361 - 380 of 662 results

  2. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...vectors is how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates...

  3. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...biomedical applications of these tools difficult or impossible. But if you’ve ever had an MRI, you know that...the self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could be expressed...

  6. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... for every potential new target and basically impossible if you wanted to distinguish between endogenous... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

  7. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...course of the last few years: recognizing the importance and benefit of remote and video communication...staff have become a new way to allow you to get important information about the campus even if you can’t...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...suffers from several limitations. Perhaps most importantly, most Cas9 orthologs are less well characterized...target site. Benefits of truncated gRNAs Importantly, the use of truncated guides has largely been...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...chromoproteins and fluorescent proteins, it’s important to note that despite their distinction by name...interactions with cytosolic proteins. This step is important as non-specific binding to proteins can inhibit...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...expression. Despite their ease of use, it is important to note that bacteria usually cannot produce functional...proteins are assembled in vitro using purified components of the transcription and translation machinery...

  11. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ... prevalent in human cancers, and synthesizing compounds to bind specifically to their mutated residues...community, will soon expand to include several other important pathways. We invite feedback and contributions...

  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...mentor and graduate office. I cannot stress how important they were to my success as a student. They didn...and more voices of encouragement emphasize the important roles of scientists outside of academia One recent...

  13. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...

  14. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...have that conversation out in the open is really important. Addgene: Is there anything else you’d like to...biology is massively underfunded considering how important it is. I would really like to reach all those ...

  16. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13 enzyme fused to an RNA adenosine... Abudayyeh, Omar O., et al. "C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector...

  17. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ... increasingly politicized. It would be nearly impossible for a career scientist such as Zimmer to introduce...self-promotion, and personality is playing an increasingly important role in science. The science produced by the quieter...

  18. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...gene-expression sequences, and more. By combining these components, one can generate constructs to visualize cell...facilitate the construction of plasmids with many components. The creators of the MXS-chaining method have...

  19. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ... step, you’ll want to ensure that your buffer components are compatible with your conjugate and conjugation...immunohistochemistry, and immunocytochemistry. It’s important to note that some antibodies are better suited...

  20. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...experiments didn’t provide any information on other important factors such as what is the best promoter to use...successfully replicate published works. Of particular importance to these efforts is the open sharing of negative...
Showing: 361 - 380 of 662 results