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We narrowed to 745 results for: nes

Showing: 361 - 380 of 745 results

  1. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...that’s considered unlikely to change – but the illness has been fatal in 70 percent of the most recent...infection also makes it a critical component of vaccines and the target of neutralizing antibodies and ...Ebola representing the latest outbreak and earlier ones, along with links to the relevant scientific literature...

  2. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ...My son is an engineer and my daughter has a business degree. From the very start of their undergraduate...of driving systemic change in academic career readiness training. It's been an honor to work with such... essential that we integrate inclusive career readiness training in this educational path before we lose...

  3. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...to knock-out single genes in clonal populations and to knock-out thousands of genes in mixed population...targeting over 19,000 and 20,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone...

  4. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...the CRISPR/Cas9 system to precisely edit specific genes in monkeys. "Our study shows that the CRISPR/Cas9...system enables simultaneous disruption of two target genes in one step without producing off-target mutations...the news on CRISPR has gotten, consider these headlines in The New York Times and Forbes respectively:...

  5. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ... the biomedical research community. Along these lines, Darrell recently joined the Addgene Board of Directors... various lung diseases. Chapter 10: How Darrel defines Open Science and how he practices and promotes ...open science in the lab - sharing reagents, cell lines, data, and knowledge without exclusivity. Chapter...

  6. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens...in line with current Web Content Accessibility Guidelines (WCAG). These changes mean our content is both... Chonnettia Jones...

  7. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...culture of engagement in the issues at hand and eagerness to get, as well as give, information to make improvements...to the manager. Team members rarely rush to give honest feedback to the manager for a variety of reasons... frequency. Anonymity can be a great way to get honest feedback, but shouldn't be the only way.   From...

  8. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...perform pooled CRISPRi screens to identify essential genes and phage host factors, and identify sequence-specific...Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis of human metastasis cohorts...knockout library targeting mouse tumor suppressor genes (TSG). Find the Mouse Metastasis CRISPR-Cpf1 Double...

  9. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ... more readily enable research. Often “normal” timelines that could be weeks to months were reduced to ...Institute                                       Chinese Academy of Medical Sciences University of Texas...Stanford University University of Iowa University of Minnesota See highlights from the COVID-19 collection Partnership...

  10. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ... cell’s surface proteins and this interaction determines the specificity and efficiency of the AAV serotype...each approach and to follow best practices and guidelines. It was wonderful to see the development of this...by the workshop participants  - a testament to openness and sharing in science.  We’re excited to support...

  11. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...and can deliver large sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas...systems; Cas12k in Type V-K systems; Cascade shown) combines with a donor template and transposon proteins ...Prime-Editing-Assisted Site-Specific Integrase Gene Editing), which combines prime editing with a site-specific recombinase...

  12. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...able to obtain eight clones. We ran digested and undigested samples of the clones on an electrophoresis... and I were unsure if there were any successful clones because the bands were all in the wrong place. ...

  13. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Targeted manipulation Controlling stimulation Invasiveness  Choosing the best set of tools therefore depends...precise and the offset of stimulation is gradual. Invasiveness Both optogenetic and chemogenetic tools need...region Impacts all cells expressing receptors Invasiveness Mice require permanent intracranial implant ...

  14. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... may be electrochemical or light-based, with luminescent and fluorescent reporters being especially popular...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

  15. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...easy to spot meeting participants. They were the ones holding poster tubes (or as our baffled flight attendant...specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the academic...changing it from a puzzle to decrypt to a tool to be harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's...

  16. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...it takes to be a good scientist are not the same ones it takes to be a competent manager (much less a ...strong sense of engagement in the work Behind-the-scenes support from their bosses and employers. Availability...may not necessarily be comfortable with imposed deadlines (there’s that need for flexibility, sometimes ...

  17. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...Vectors (or empty backbones) are frequently used in molecular biology to isolate, multiply, or express...At Addgene, we have a vast collection of empty backbones that have been designed, tested, and published...vector). Check out our complete guide to empty backbones for additional details and guidance for choosing...

  18. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...discards according to the Cambridge, Massachusetts guidelines and wear an extra sweater so we don’t have to...visualization or isolation, then using only a few lanes or a small portion of the gel.  What a waste?  I... pour over the sides.  These tiny gels and tiny lanes required less agar, less ethidium bromide, less ...

  19. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... HEK is it good for?   As one of the older cell lines in the research community, many cellular research...organism. This pipeline is utilized to transduce cell lines for basic science research all the way up to the...HEK is wrong with it?   All cultured human cell lines are susceptible to contamination by mycoplasma and...

  20. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...20th century, and many iconic papers, including the ones on the structure of the DNA, were not peer-reviewed...submitted paper in addition to the journal-specific guidelines.  Peer-review step 1: The first read At the first...preferably the following morning - as usually deadlines are pressing. During this second read, I pay more...
Showing: 361 - 380 of 745 results