Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 628 results for: rel

Showing: 361 - 380 of 628 results

  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...protocol link). We have found this method to be reliable and reproducible. However, we do not use amplicons...which does not a standard curve and somewhat more reliable quantitation. Differences in sample treatment ...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...are some of the AAV and lentiviral preps we have released in the last few months: Lentiviral preps for ...coming soon! These vectors listed below should be released at Addgene in the next 6 months, pending quality...

  4. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...specific goal for each analysis will help you locate relevant information in the future. Approach: Outline...can store any power point, excel, or other files relevant to the project. README: The README.txt document...

  6. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... and how these pathways work, as this could be relevant when planning your experiment. Here, we’ll discuss...repair is that the Cas9 protein doesn’t immediately release from the broken end after cleavage, which may interfere...

  8. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...biology. Plant molecular biology has traditionally relied on overexpression and knockouts to better understand...technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential...

  10. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...bacteria of clinical significance and four clinically relevant host biomarkers. The lab validated their platform...called distributed cell division counting (DCDC), relies on the assembly of fluorescent proteins into one...

  12. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...built to protect us now seems to attack us by releasing mitogenic growth mediators and improving cancer...resources on the Addgene blog Read our cancer related blog posts Learn about our cancer collection pages...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...Mbp, with the calculated fluorescent distances correlating with the previously established physical map....

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...and our AAV data hub to see if others have used relevant viruses for your system. This post was contributed..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...

  16. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog...improve gene editing performance at therapeutically relevant gene targets and expand the application to the...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...As with DNA isolation, scientists commonly rely on RNA isolation kits to make their life easier. Recently...resources on the Addgene blog Read blog posts related to plasmid protocols Find protocols for purifying...

  18. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ... one of them. Why might the sex of the cell be relevant? Research shows that there are sex differences... of cells and tissues—when that information is reliable—is important to ensuring excellence in basic research...

  19. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first discovered... destination vector + insert may spontaneously religate, this transient construct retains functional Type...

  20. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...attention to the keywords used and think of ways to relate the proposal and deliverables to the aims requested... technologies! If data from a publication or preliminary data suggests the feasibility of the statement...
Showing: 361 - 380 of 628 results