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  2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...built to protect us now seems to attack us by releasing mitogenic growth mediators and improving cancer...resources on the Addgene blog Read our cancer related blog posts Learn about our cancer collection pages...

  4. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...Mbp, with the calculated fluorescent distances correlating with the previously established physical map....

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...As with DNA isolation, scientists commonly rely on RNA isolation kits to make their life easier. Recently...resources on the Addgene blog Read blog posts related to plasmid protocols Find protocols for purifying...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog...improve gene editing performance at therapeutically relevant gene targets and expand the application to the...

  7. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...and our AAV data hub to see if others have used relevant viruses for your system. This post was contributed..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...

  8. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ... one of them. Why might the sex of the cell be relevant? Research shows that there are sex differences... of cells and tissues—when that information is reliable—is important to ensuring excellence in basic research...

  9. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...cloning efforts. Golden Gate cloning technology relies on Type IIS restriction enzymes, first discovered... destination vector + insert may spontaneously religate, this transient construct retains functional Type...

  10. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...attention to the keywords used and think of ways to relate the proposal and deliverables to the aims requested... technologies! If data from a publication or preliminary data suggests the feasibility of the statement...

  11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...particularly useful for understanding a specific effect related to an experimental manipulation. Common inbred ...Mice are fed or injected with tamoxifen, which relieves natural inhibition of the Esr1 domain that blocks...

  12. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...socioeconomic or educational background, cultural or religious affiliation, and ethnic or personal identity. ...symposia, or courses in social, health or policy-related topics, as time permits. Also seek out opportunities...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...human glycosyltransferase genes and other proteins related to cellular glycosylation. Get the library at ...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... from Ashley et al., 2021.   The AID system relies on a transport inhibitor response 1 (TIR1) expressed...induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner’s lab...

  16. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...expand already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed....8b00333 Pryor, J. M., Potapov, V., Bilotti, K., Pokhrel, N., & Lohman, G. J. S. (2022). Rapid 40 kb Genome...

  17. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  18. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...restriction enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to ...reducing the culture volume into which the virus is released, a more concentrated virus solution can be obtained...

  20. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...research goals in focus. Developing these skills relies upon biologists and computer scientists learning...neuron knockouts so that we can perturb factors related to worm motion. Working on the Cat Microbiome Project...
Showing: 361 - 380 of 617 results