We narrowed to 821 results for: LAS

...once in the plasmid backbone and another enzyme that cuts within the insert. If the plasmid contains the... PCR product if the plasmid contains the insert and no PCR product if the plasmid does not contain the...resources on the Addgene blog Get all the plasmid basics in our Plasmids 101 series Find more molecular biology...If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone...clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to begin ... how to use positive and negative selection in plasmid cloning.  But oftentimes, a selection strategy...enough on its own to help you find your desired plasmid. In many cases, you will need a screening strategy...

... Bioblasts? Plasmagenes? In the 1940s and 50s, scientists were working to understand genetic cytoplasmic...how did plasmids get their name? In 1952, Joshua Lederberg set out to clarify the classification of these...these cytoplasmic inheritance factors. He proposed the catch-all term “plasmid” derived as a hybrid of ...tetracycline resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI...engineering, cloning, and plasmid tags, addtional content added to the "Plasmids that Glow" and "Viral Expression...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua...of "cytoplasm" and "id" (Latin for 'it'), as “a generic term for any extrachromsomal hereditary determinant...

...letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is for plasmid. pXXXXX-XXXX...information about plasmid features and tips you can use in the lab, check out more posts in our Plasmids 101 series...There are no universal rules for naming plasmids but here are some good guidelines to follow in order...people can quickly and easily identify what your plasmid contains and other important information. Tip:...Backbone name Include the empty backbone name in your plasmid name. This simple piece of information can often...important details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial...  Include information about the insert in your plasmid name. This is often a 3-6 letter representation...

...2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017 for all incoming plasmids was a...every isolated plasmid sample and normalize each to fall within a 10 fold range. Plasmids that don't meet...A broader view of the whole plasmid To confirm the sequence of the plasmid, we examine three things: ...function of the plasmid.   Confirming the insert We usually confirm the insert through BLAST or by direct...expected and do not affect plasmid function, we will update information on the plasmid’s page. Sometimes we’re...’re missing a piece of the plasmid There are regions of some plasmids that are particularly difficult ...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are ...

...the plasmid based on the predicted sizes and organization of different features within the plasmid. Restriction...if you don't have the full plasmid sequence. Once you have purified plasmid DNA, this method can be done...example plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert. The plasmid was digested...possible plasmid conformations, with relaxed and nicked marked with asterisks (*). When the plasmid is digested... Congratulations, you have a plasmid expressing your gene of interest (YGOI) and are ready to dive into...functional experiments! Whether you’ve cloned the plasmid yourself or obtained it from a colleague down the...with the correct construct, and verify that the plasmid you received matches the expected sequence. Here...

... metabolic enzymes including biotin carboxylase and decarboxylases. These enzymes catalyze the transfer...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...

... on All Things Plasmid with Our Plasmids 101 Series Get Tips on Verifying Your Plasmid Resources on Addgene.org...Check out Our Plasmid Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes to add... Interest (YGOI) for ligation into a recipient plasmid. Designing primers for PCR based cloning   ...this example). Assuming you are amplifying from plasmid DNA (rather than from genomic DNA or a cDNA library...

... (blue) light switch. Zhang’s team devised the plasmids now available in Addgene’s repository for use ...biological processes and disease states. While LITE plasmids have clear application to anyone with an interest...the new optogenetic tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the...

...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...future improvements. Find PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024).... fat cells fight cancer Frugal CRISPR kit for classrooms Cell-surface proteomics for neurons and astrocytes...isolated, identified by mass spectrometry, and classified by source cell based on single-cell RNA-seq data...cell types, and tissues. Find cell-surface HRP plasmids here! Wu, L., et al. (2025) The cell-surface ...systems, both in vitro and in vivo. Find MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023... al. (2025) under CC BY license. With 143 plasmids deposited from this paper, a large menu of tool...

...applications. Find ATLAS plasmids here! Rivera, J. F., Huang, H., et al. (2025). ATLAS: a rationally designed...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles... and AAV11 packaging plasmids Mapping anterograde synaptic connections with ATLAS Bright and photostable...Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ATLAS for anterograde synaptic connections ...fluorescent protein mGold2 By Mike Lacy Get your sunglasses — these proteins are bright! Most current yellow...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...