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...replication components are called helper plasmids. Below, we’ll breakdown the vector components first by ...there are bonus components to these vectors that aren’t reflected in the common components outlined above...lentiviral guide outlines these components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated...production reasons, multiple plasmids with many components are used to package lab grown viruses. It can... protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and their... what gag and pol stand for, we demystify all components of viral vectors here! Need Virus? Check out...adenovirus, and adeno-associated virus (AAV). The components of these vectors are also split across several...

...through two sequential homologous recombination events: The plasmid carrying the desired genetic modification... Partial Characterization of the Pink and Blue Pigments of Pocilloporid and Acroporid Corals. The Biological...G, Kühl M, Hoegh-Guldberg O (2000) Fluorescent pigments in corals are photoprotective. Nature 408:850–...

...errors in the design of cloning strategies set experiments back days or even weeks. Primer design was done...principles. For every task, we envision what the user wants to do and make the path to accomplishing their goals...is an ongoing process that involves iterative refinements in response to customer feedback. An example ... and display frequently used genes and control elements. Development of this tool required creating a ...variant of a feature. Instead, we identified common variants, and then crafted a detection algorithm that tolerates...sequence has changed. Identifying plasmid control elements Coding sequence features are relatively straightforward...straightforward to define, but for control elements such as promoters and transcription terminators, the boundaries...

... the field of grants. What is a grant professional?  In the past 5-10 years, the Grants Profession has...collaborations, and commitments to and proof of compliance with the grantor’s requirements as well as state... for grants, the amount of grant funds available and the increasing regulatory process for grants has ...grant consultants who offer grant training, mentoring and coaching services, including Smartegrants, to ...choice exam.  The bottom line on being a grants professional The Grants Profession is in a rapid growth phase... Inc., a national grant consulting firm, and Smartegrants, a grant professional development firm The path...established grant professionals is learning to succeed in grants through trial and error, through winning and losing...

.... Now is the time to make bold statements. While some of the statements may be slightly far reaching, ...consider the reviewer's comments. Whether the proposal is funded or not, the comments help gauge room for ...government and academic institutions. New graduate students may assume that their advisor alone applies for... allot some if not all grant duties to trusted students. For example, if the proposal call aligns with...write a grant!”, “I don’t have time for this if he wants data by next week!”, and “This is his job!” filled...various skill building opportunities that graduate students and postdocs should actively pursue. Proposal ...italic, or underline font formats helps these statements stand out from the rest of the text. Specific...

...single 96-well plate for HDR that satisfy all requirements. To alleviate this bottleneck, we began building...whole or in part. For example, you can analyze experiments designed outside CrispyCrunch, or you can input...step of mutating guide sequences in a way that prevents re-cutting but does not disrupt gene expression...that worked with NGS data were: Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca...re-create a match for the active guide. With these constraints in mind, we implemented the following algorithm...Design donor RNA Design primers Review and order reagents Perform your wet lab experiment Analyze the data...high-throughput design and analysis of CRISPR experiments. It has certainly boosted our productivity in...

...(i.e. the plasmid complements the deletion). If the phenotype is corrected, students can be relatively... multiple enzymatic steps (5). In their experiments, students test whether or not proteins from the fission...extensive rearrangements in the S. cerevisiae lineage (7). In our class research project, students transform...in other experiments (Figure 2).   Complementation reveals conservation of function Students have used...Boston College. Students in the course work in teams of three in sections of 15 students. Within each section...also used to teach students about restriction mapping and western blotting. Students who participate in...authentic research experiences to undergraduate students (1, 2), but educators face significant challenges...

...levels and affect behavior. A fact to consider: Rodents can hear ultrasonic frequencies (> 20 kHz, produced...

...around the globe, we’re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, ...

.... Both awardees emphasized the contribution of students in their labs and the importance of the strong... what can be done with this technology to help patients. Dr. Stoffels and others in the audience discussed...technologies. Addgene is proud to be helping get CRISPR reagents out to academic labs. It is our hope that robust...