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We narrowed to 48 results for: codon optimized

Showing: 11 - 20 of 48 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...series of blue, green, and red FPs that are codon optimized specifically for expression in yeast. These...—in frame, of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (...Based on their findings, the authors recommend optimal FP combinations for use in yeast imaging, categorized... output requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or...

  2. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...of gene synthesis, many researchers now use codon-optimized versions of common coding sequence features...genes, detection needs to occur even if one or two codons are missing at the beginning or end of the feature...

  3. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they achieved much ...the host plant genome. Scientists have worked to optimize this system for gene transfer, studying the stability...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...with proper protein folding, and deal with sub-optimal codon usage (Table 1) A few mutations are common to...translation; contains 7 additional tRNAs for rare codons not normally used in E. coli. Expression vector...many proteins, a rich media such as TB or 2XYT is optimal because of the high cell-density they support; ...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...and this is likely a rate limiting step, but optimization of the exon-splitting site sequence can enhance...and acceptor sequences were tested and with the optimal sequences, the group achieved ~80% of muscle cells...level of transgene expression, and may need to be optimized for each unique application (Trapani et al., 2015...protein. This can be avoided by excluding a stop codon from donor A in any of the reading frames downstream...That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–2261 . https://doi.org/10.1126...

  6. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...post-translational modifications and polymorphisms Codon optimization tool: To access this tool, set up an IDT ...estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD): This tool designs overhang...

  7. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...reverse transcriptase with a sequence optimized for human codons; additional nuclear localization sequences...heteroduplex     ✓ ✓ ~ ~ ~ Human codon optimization Improve prime editor expression ~ ~...everything — prime editing typically needs to be optimized for each application, and the efficacy varies ...precise and versatile, but it requires careful optimization to achieve the best efficiency. The type of ...efficiency can vary widely — so its inventors kept optimizing it. Using a pooled CRISPRi screen, the Liu lab...originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor sequence to improve expression and...as prime editing, every efficiency counts! The optimized enzyme can be used with any of the PE2-5 approaches...

  8. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... a few months. More time is required for its optimization and development before it can truly be pitted...PAM sequence might not always be available at an optimal distance from the target site. For example, NGG...AT-rich regions of the genome or just after a stop codon (as required for C-terminus epitope tagging). NgAgo...method and 65% wanted to wait until someone else optimizes an NgAgo protocol for their favorite model system...respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian cells. However...indicates that it is very tricky to use and fussy to optimize. One reason could be the instability of the supposedly...Verdict", is that more time is required for NgAgo optimization before we can make any major conclusions: That...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...cassette into a given locus, just upstream of a stop codon. Briefly, the PITCh vector should be constructed...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...GFP-Puro will be inserted just upstream of a stop codon. One potential concern is if the GFP-Puro will alter...

  10. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...Furthermore, we did not find any tools which were optimized for HDR, except for TagIn. HDR requires the additional...enabled it to return guides and primers in batch, optimized for HDR. Compared to wealth of resources for guide...insert, the more poorly the guide performs HDR. Optimal mutation in-depth One the most sophisticated features...mind, we implemented the following algorithm for optimal mutation in CrispyCrunch: If the guide PAM resides...above are options, the algorithm silently mutates codons in the guide sequence, one by one, from the PAM...
Showing: 11 - 20 of 48 results