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We narrowed to 48 results for: codon optimized

Showing: 31 - 48 of 48 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human cell line expression...provided in both open (no stop codon) and closed (including a stop codon) formats. The collection is compatible...directly for drug screening or easily shuttle the optimized, expression validated GPCR sequences to any desired...strains remains seriously limited, as prior optimizations are required for each individual target species...being generated, there is a need for genetic optimization of industrial yeast strains. However, industrial... the well-known yeast two-hybrid assay are not optimal for studying native mammalian protein interactions...transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila and yeast...

  3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...less efficient than Cre recombinase. FLPe codon optimization resulted in FLPO, which shows similar efficiency...loxP sites (For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different FLP...first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient in mammalian...identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart 1998)....

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...talk about western blot optimization and troubleshooting.   Before you can optimize or troubleshoot a western...backgrounds.  Once you’ve optimized antibody concentration, you can optimize ‌incubation conditions to...want to spend optimizing it. Blots for quantification generally require more optimization than blots used...with running a gradient, which will allow you to optimize the concentrations of your various blot reagents... a Western Blot post to make sure you have the optimal gel for your experiment.   Ladders If you need ...trickier than gels and may require multiple runs to optimize or troubleshoot.   Before you start… Mark your... semi-dry are not working, you’ll want to try optimizing your transfer buffer.  Adjusting the buffer For...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and ...issues as traditional luciferases emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require... emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution is to couple ...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors. The system ...follow these same assembly principles, but they’re optimized for different purposes.   Gersbach Lab multiplexing...destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids are available for both monocot and... paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for efficient germline and somatic...Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE Recombineering." Sci Rep. 6:19452. PubMed...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest to...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

  13. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...develop an optimized photoactivatable CRISPR gene activation system, but settled on a different optimal fusion...researchers tested a variety of combinations to optimize both fusion proteins. The best performing combination...fusion protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the... would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...

  15. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... to the use of exon skipping, alternative start codons, and/or the phenomenon of transcriptional adaptation...and robust gene silencing (following initial optimization of the amiRNA). Although downstream work is ...

  17. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...synaptic glutamate response. They generated variants optimized for different applications (v82 has higher affinity...indicator (GEVI) called JEDI-2P (Liu et al. 2022) optimized for two-photon imaging. They use a unique and ...multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors...variety of imaging conditions (155 μs and <0.1 °C in optimal conditions). While B-gTEMP itself is a significant...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators ...

  18. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max with a ...cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature Biotechnology...
Showing: 31 - 48 of 48 results