...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds ... This selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each...plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References...

..., plasmid kits are avaliable for less than than it would cost to buy each plasmid in the kit individually...This describes how the plasmid or other product (kit, pooled library, etc) will be sent to you. Most of...

... tools that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...biological systems” as they predict. The pOSIP plasmid kit can be found at Addgene, so what are you waiting ...constructs, while the Patron Lab MoClo Plant Parts kit contains modules specific for plant transformation... For further reading about Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview...annealing (SSA) assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces...additional information on using the Platinum Gate TALEN Kit, please see the Yamamoto lab’s protocol for TALEN...specifically to improve plant transgenesis. TheGreenGate kit available at Addgene includes six individual types...

...and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized....1016/j.neuron.2024.08.019.  ONE-GO biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) ... of selectivity. With 10 plasmids available as a kit, plus 21 optional accessory plasmids, the collection...

...plasmids, ready-to-use viral preps, pooled libraries, kits, and cloning grade DNA!     This is just the first...

...topoisomerase (2).  These days commercially available TOPO kits provide vectors or cloning arms with overhanging...covalently linked to topoisomerase. Vectors in these kits also often have the topoisomerase site inserted ...

...of the PCR reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready...

... the entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great ...great information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter...

...Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...

...The 4 kits also contain the two entry vectors pUC-L1L4 and pUC-L3L2. Find the 2in1 plasmid kits at Addgene... Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different, binary...

...done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish ...that can be activated to emit light with a simple kit, and is readily available on any number of secondary...

...little more colorful? CRISPRainbow is available as a kit from Addgene.   References 1. Chen, Baohui, et al...Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags...

...looking to conjugate antibodies yourself, there are kits available to make the process quick and easy. Before...antibody’s buffer is compatible with your conjugation kit, since many common antibody buffers and buffer additives...

... can be done in-lab using commercially available kits or ordered as synthesis modifications when purchasing...choose from and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label...

...Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA. This technique works by...interested in detecting in your sample. When an ELISA kit is ordered, the wells are typically already coated...

...unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta...

...there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of hours...day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted to...

...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...

... you prepaid shipping materials (i.e., a deposit kit) with instructions on how to send liquid DNA or bacterial...pages for your review. We’ll also mail you a deposit kit with instructions on how to prepare and ship the ...

... colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields...lead to degradation of your prep. Many plasmid prep kits recommend performing an extra wash step to remove...

...and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and every contributor... for Protein Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who wrote a ...

...Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent Protein Kits Browse the Fluorescent Protein... recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging...offers for tagging a single genetic locus, the toolkit also offers repair templates for tagging proteins... elegans, several groups have designed modular toolkits to assist with tagging genomic loci in other model...obtained as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging... S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging vectors (eg GFP... Matthew L. and Eric M. Jorgensen. “SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification...

...used in this study.  The zCas9i cloning kit is a plant cloning kit that contains a series of vectors and...

...mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found across all TCGA...Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in ...

...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting...

...Lentiviral Expression Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids...

...mycoplasma contamination using mycoplasma detection kits. Cell line is maintained for ~20 passages before...prepared using endotoxin-free plasmid purification kits.     Figure 4: Schematic of the barcode ...

...working, consider using a subcellular fractionation kit or a lysing protocol developed for isolating proteins...based lysing protocol Subcellular fractional kit or lysing protocol Not enough target Run protein...Chemilumenescence: use a more sensitive exposure kit Too much signal Antibody dilutions too high...Chemilumenescence: use a less sensitive exposure kit No signal variation in highly expressed proteins...

...Plasmids from the Ellis Lab Find Synthetic Biology Kits Browse Our Synthetic Biology Pages ...

...targeted markers. Some commercially available fix/perm kits allow for labeling of cytosolic proteins, as well...located inside the nucleus, a different fix/perm kit is required (to be precise, the fixing solution is...

...Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate ...

...in this blog post. The pDGE Dicot Genome Editing Kit from the Stuttmann lab contains a variety of Agrobacterium-compatible...from the Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the...described above, Addgene carries several useful CRISPR toolkits for creating plant expression plasmids, including...

... the paper in bioRxiv Find the GeneWeld vector kit CasX, a small Cas protein, is active in E. coli ...

... FRET biosensors can be created using the cpFRET kit created by Olivier Pertz’s lab. Other biosensors ...

... Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy. ...

...biologists bought the pricey stuff in 50 ul aliquots in a kit with a huge amount of wasteful packaging.  Turns ...

...educational company that creates hands-on science lab kits and digital curriculum to support them. Many thanks...

...integration vectors (see for example this yeast cloning kit) she intends to make yeast capable of desaturating...

... plasmids and resources Find fluorescent protein kits ...

.../reporter parts of each construct. This new tool kit allows the rapid generation of stable transgenic ...