...prep pAAV-hSyn-GRAB-gDA3m AAV1 Biosensors Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2-WPRE AAV1...

..., Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...

...Lentiviral CRISPR-Guide RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa ...

...gene is synthetic or natural? The answer to this lies in the codon usage biases of different organisms... in synthetic biology and DNA synthesis. They published their results in a paper in Nature Communications... Nielsen & Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to...

...Addgene’s traditional excellence through whatever lies ahead this year. We can see signs of the storm passing...

...to offer AAV for the newest GRAB sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal...

...Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor...and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, ...

...serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G AAV1, AAV2,...

...Second generation GRAB dopamine sensors from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h...

... with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a series of...a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls the... dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment LOVTRAP is...localization Article contributed by Tyler Ford  Listen to the riboglow podcast segment Researchers from... story Article contributed by Caroline LaManna  Listen to the mCarmine podcast segment Scientists are ...activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast segment The increasing...

... F., Conatser, L. M., Lue, V. M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated...fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (2019). ...

.../doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang...

...characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018) Labs also shows promise for in...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...

... 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted...

... E. coli strains including the popular BL21(DE3) (Li et al., 2021). This pEcCas/pEcgRNA system works similar...–1290 . https://doi.org/10.1021/acssynbio.7b00399 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021)...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...bacterial CRISPR engineering One common way of accomplishing bacterial genome engineering is done with recombineering...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...systems is base editing. This process is generally accomplished by fusing an enzyme capable of converting one...

...nar/gkw930 Yu Y, Wu X, Guan N, Shao J, Li H, Chen Y, Ping Y, Li D, Ye H (2020) Engineering a far-red light–activated... LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control... and repeatable. Since these initial studies published in 2015, more split systems have been developed...Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems.  Listen to the anti-CRISPR podcast segment Simply expressing...

... Zhu, Z., Liu, G., Kou, L., Wang, Y., Qiu, J.-L., Li, J., & Gao, C. (2021). High-efficiency prime editing...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...

...experiment! References Becher, Burkhard, Ari Waisman, and Li-Fan Lu. "Conditional gene-targeting in mice: problems...

...References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control ...

...gene that kills the cells expressing it? The answer lies in special strains of E. coli that tolerate the ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...

...References and Resources References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007) Evolving...

...why is FLEx useful? The power of the FLEx system lies in the ability to conditionally turn off one gene...optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses for FLEx switches...

...doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich L, Li C, et al. (2018) OGT binds a conserved C-terminal...

....201409074 Tran NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal ...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted...being the source of irreproducible data (Begley & Ellis, 2012; Baker, 2015). This contributes to an enormous...Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A ...276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012) Raise standards for preclinical cancer...

...and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic... trials The CRISPR therapies don’t stop with the list above! There are many, many more in the earlier ...

...expression and purification. Li J, et al. Science. 2017. PubMed PMID: 28082411 Listen to the podcast segment...increased ECT-DNA. Diving further into TZAP function, Li and colleagues observed endogenous TZAP localization...PubMed PMID: 18094608 Listen to the podcast Segement on the pPSU Plasmids B. subtilis knockout and knockdown...recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin...2016. PubMed PMID: 27238023 Listen to the podcast segment on the B. subtilis libraries Transcription factor...Cells) acrIIA1 Listeria monocytogenes  pRAU168  No  pJH372  No  acrIIA2 Listeria monocytogenes  pRAU171...   acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes...

...Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy May... Crispresso, that adhere to the same principles listed here. It employs biological best practices. For... is generally advised, there is little detail published on how to do it best. Scientists are typically...

...range 6.0 (yellow) - 8.0 (red)). Blood pH normally lies around 7.35-7.4 so mammalian cells are sensitive...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...

...Addgenies has helped me get a much better idea of what lies ahead. Thus the “Meet an Addgenie” Instagram interview...choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told...don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped me realize that is...Scientists are often under a lot of pressure to publish their research and are not so keen on sharing their...came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the most purpose at Addgene...

...hyperpolarization. PSAM ligands The advantage of PSAMs lies in their ability to be specifically and potently...19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic...

... genome editing can help accomplish this goal. The power of this system lies in the ability of the endonuclease...

... CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

...become clear that the power of this editing platform lies in the diversity of nucleases and their applications...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

...affinity purification with an anti-Cas9 antibody, the Li lab found a microRNA (miR483) that binds to the promoter...

...PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation...

...interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021 Yujie Sun ...

...in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular...whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D...

...13226-x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D....

...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...

...PubMed Central PMCID: PMC1462937. Dietzschold, B., Li, J., Faber, M., and Schnell, M. (2008). Concepts ...Sorensen, S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K....advantage of AAV vectors is that there are well-established protocols for their production and purification...AAV1, -2, -5, -7, -8 (Taymans et al., 2007), AAV1 (Hollis et al., 2008) AAV6 (Towne et al., 2010), AAV8, ...PMID: 19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R...