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  1. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...~300-500 ColE1 (derivative) and F1** A Relaxed pGEM ~300-500 pUC and F1** A Relaxed pCDF ~20-40 CloDF13...Plasmids with phage-derived ori's are referred to as phagemids.   Other factors that affect plasmid copy number...
  2. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...pertinent to the research community, two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the...  Resources on the Addgene Blog Download the Management for Scientists eBook Download the Plasmids 101...
  3. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...away from one man teams.  The composition of the management team is more important than the product itself...essential component to any successful startup management team). Sreyan is a research associate at Harvard...
  4. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...to write.  Pomodoro technique timer  One time management technique that you can use as part of your writing...PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique": This podcast talks ...
  5. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...mentoring. Some mentees prefer lots of advice and micromanagement, while others are more productive in a handsoff... Right Moves: A Practical Guide to Scientifıc Management for Postdocs and New Faculty" downloadable book...
  6. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...technical and transferable (i.e. teamwork and time management), your ability to solve problems efficiently ...with others outside of your organization Time management Managing a team Leadership Resources on Addgene.org...
  7. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...career preferences: levels, changes, and advisor encouragement." PloS one 7.5 (2012): e36307. Pubmed PMID: ...Learn how to be a successful manager with our Management for Scientists Series  ...
  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...
  9. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...introduction, methods, results, conclusions, and acknowledgements sections. Some other additions you might wish...the introduction, methods, conclusions, and acknowledgements sections are inserted. The advantage of this...
  10. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...and concerns of these communities. This kind of engagement and two-way dialogue is crucial if we as a society...worked with a number of organizations in public engagement efforts, including giving public lectures, writing...
  11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...fragments that are efficiently subcloned into the phagemid pDX_init plasmid, using the fragment exchange ... restriction sites (Geertsma, 2013). Once the phagemids are constructed, they are used to produce a phage...
  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...coffee with potential new friends. Without the encouragement and pep talks from these contacts, I would have...Scientific Training More and more voices of encouragement emphasize the important roles of scientists ...
  13. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...next to you. There is an active Twitter scene of iGem teams that is really useful also. There is also ...reaching senior positions. There has been a lot of engagement with the Athena SWAN Charter and it has really...
  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...etc… Low (except for space) Real-time audience engagement and response. High connection levels and interaction...geographic location) Opportunity for real time engagement while allowing for recording/future access. Remote...
  15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...that are scanned into our Laboratory Information Management System (LIMS), a database we use to keep track...   Check Out How We Think about Developing Lab Management Software    The plasmid's journey begins First...
  16. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...accessible. The supporting data and community engagement required to do this drove Addgene to develop ...partnerships, and new platforms, from our early engagement with UPenn to our launch of the redesigned Data...
  17. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...Imagine you’re applying to a job with a lot of file management that asks for strong organizational skills. You...Professional Network Become A Good Manager with Our Management for Scientists Series Get Tips on Finding Your...
  18. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...Addgene I am now involved in Strategic Partnership Management, a very interesting and exciting part of my work...and for training in teamwork and collaboration management. For example, you may already be part of a scientific...
  19. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...analyze. Criteria for a bioinformatics workflow management system As novices to creating a robust data pipeline...engineering pipelines, we selected Nextflow, a workflow management system for scientific pipelines, including bioinformatics...
  20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ..., combining data, team, inventory and project management functionalities with experiment records. Many...reproducibility 5 great apps for lab life Developing Lab Management Software for Biology Resources on the Addgene...
  21. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...approach is to interrogate state with qPCR. The re-arrangement of DNA segments in each state of a register enables... of a set of chemicals. Specific examples of arrangements that should be able to record all permuted substrings...
  22. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... problems can, of course, be solved with good management practices, but can sometimes go unnoticed at ...project to validate some genomic changes, so this arrangement was ideal for everyone.  The cutting edge science...
  23. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...needs, our criteria for selecting a new workflow management system, and how Nextflow helped us manage our...
  24. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like to give a shoutout to BosLab...
  25. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...functional, handling everything from facilities management to website development, tech transfer, lab work...
  26. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Diplomacy, with a focus on international technology management, from the Fletcher School at Tufts. From 2004...
  27. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...out including the single base editors, Casilio, CombiGEM, and a variety of pooled libraries. Not all of...
  28. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...material in human subjects or clinical trials Acknowledgement in recipient’s future publications relating...
  29. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... Nature News & Views Andreas Möglich and Peter Hegemann described several advantages of the new system...
  30. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...available.” Choosing a research project “entails good judgement with the current technology to identify a problem...
  31. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ... safety features provide extra layers of risk management when working with viral vectors. Typically, one...
Showing: 51 - 100 of 173 results