We narrowed to 619 results for: LIS

... with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a series of...a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls the... dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment LOVTRAP is...localization Article contributed by Tyler Ford  Listen to the riboglow podcast segment Researchers from... story Article contributed by Caroline LaManna  Listen to the mCarmine podcast segment Scientists are ...activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast segment The increasing...

.../doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, Guo L, Guo R, Chen T, Hu J, Xiang...

...characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018) Labs also shows promise for in...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...

... 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted...

... Zhu, Z., Liu, G., Kou, L., Wang, Y., Qiu, J.-L., Li, J., & Gao, C. (2021). High-efficiency prime editing...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...

... E. coli strains including the popular BL21(DE3) (Li et al., 2021). This pEcCas/pEcgRNA system works similar...–1290 . https://doi.org/10.1021/acssynbio.7b00399 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021)...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...bacterial CRISPR engineering One common way of accomplishing bacterial genome engineering is done with recombineering...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...systems is base editing. This process is generally accomplished by fusing an enzyme capable of converting one...

...nar/gkw930 Yu Y, Wu X, Guan N, Shao J, Li H, Chen Y, Ping Y, Li D, Ye H (2020) Engineering a far-red light–activated... LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control... and repeatable. Since these initial studies published in 2015, more split systems have been developed...Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems.  Listen to the anti-CRISPR podcast segment Simply expressing...

...References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control ...

...gene that kills the cells expressing it? The answer lies in special strains of E. coli that tolerate the ...take up your insert. But exactly how does ccdB accomplish this? Let’s start with a brief history of the...

...experiment! References Becher, Burkhard, Ari Waisman, and Li-Fan Lu. "Conditional gene-targeting in mice: problems...

... F., Conatser, L. M., Lue, V. M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated...fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (2019). ...

...References and Resources References Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007) Evolving...

...why is FLEx useful? The power of the FLEx system lies in the ability to conditionally turn off one gene...optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses for FLEx switches...

...doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich L, Li C, et al. (2018) OGT binds a conserved C-terminal...

...and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic... trials The CRISPR therapies don’t stop with the list above! There are many, many more in the earlier ...

....201409074 Tran NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal ...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted...being the source of irreproducible data (Begley & Ellis, 2012; Baker, 2015). This contributes to an enormous...Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A ...276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012) Raise standards for preclinical cancer...

...expression and purification. Li J, et al. Science. 2017. PubMed PMID: 28082411 Listen to the podcast segment...increased ECT-DNA. Diving further into TZAP function, Li and colleagues observed endogenous TZAP localization...PubMed PMID: 18094608 Listen to the podcast Segement on the pPSU Plasmids B. subtilis knockout and knockdown...recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin...2016. PubMed PMID: 27238023 Listen to the podcast segment on the B. subtilis libraries Transcription factor...Cells) acrIIA1 Listeria monocytogenes  pRAU168  No  pJH372  No  acrIIA2 Listeria monocytogenes  pRAU171...   acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes...

...range 6.0 (yellow) - 8.0 (red)). Blood pH normally lies around 7.35-7.4 so mammalian cells are sensitive...secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need to be split...

...Zuckerberg Biohub Manuel Leonetti Ryan Leenay Jason Li Max Haeussler Luca Pinello Kendell Clement Andy May... Crispresso, that adhere to the same principles listed here. It employs biological best practices. For... is generally advised, there is little detail published on how to do it best. Scientists are typically...

...hyperpolarization. PSAM ligands The advantage of PSAMs lies in their ability to be specifically and potently...19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic...

... genome editing can help accomplish this goal. The power of this system lies in the ability of the endonuclease...

...Addgenies has helped me get a much better idea of what lies ahead. Thus the “Meet an Addgenie” Instagram interview...choice. I spoke with our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told...don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped me realize that is...Scientists are often under a lot of pressure to publish their research and are not so keen on sharing their...came from Lucy Hammer, our Financial Operations Specialist. She said, “I’ve found the most purpose at Addgene...

... CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods. Researchers...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

...become clear that the power of this editing platform lies in the diversity of nucleases and their applications...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

...13226-x Cameron, P., Coons, M. M., Klompe, S. E., Lied, A. M., Smith, S. C., Vidal, B., Donohoue, P. D....

...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...

...PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation...

...affinity purification with an anti-Cas9 antibody, the Li lab found a microRNA (miR483) that binds to the promoter...

...in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular...whole-brain reference atlas efforts, including a list of validated antibodies, on their webpage: mAb3D...

...PubMed Central PMCID: PMC1462937. Dietzschold, B., Li, J., Faber, M., and Schnell, M. (2008). Concepts ...Sorensen, S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K....advantage of AAV vectors is that there are well-established protocols for their production and purification...AAV1, -2, -5, -7, -8 (Taymans et al., 2007), AAV1 (Hollis et al., 2008) AAV6 (Towne et al., 2010), AAV8, ...PMID: 19098898. PubMed Central PMCID: PMC2895694. Hollis, E. R. 2nd, Kadoya, K., Hirsch, M., Samulski, R...

...plasmids here! Zhong, B. L., Elliot, J. M., Wang, P., Li, H., Hall, R. N., Wang, B., Prakash, M., & Dunn, ...and in ternary complexes with other proteins, establishing CRBNmidi as a promising tool for future structure-guided...

...G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil...strategy Figure 4 shows a gating strategy from a published experiment (Barlow-Anacker et al., 2017). This...

...Engineering Approaches with the CRISPR/Cas9 System. Li, K et al. PLoS ONE 9(8); (2014). PubMed. Making...

...saturate the detector. In between these extremes lies the linear detection range. Figure 4: The...

...-4  Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F (2014) In vivo interrogation... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...

...academic research environment. The “how many?” question lies at the base of all these questions as there is not... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...Outcomes University websites do not currently publish career outcomes for PhDs or postdocs. Having this...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they... PIs and all gave advice on how to succeed in publishing in the current academic environment. Some great...writing skills will help you in your academic publishing goals and make you a better communicator overall...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion...

...luckily, the vast majority of my cells of interest lies within a clear negative population. When you’re ...

... Clark, A. B., Spinner, A. D., Lazzarotto, C. R., Li, Y., Matsubara, A., Urbina, E. O., Dahl, G. A., Correia...case, however, and recent work has already been published on improving base editing precision. Similar treatments...

...PMID: 25514926. PubMed Central PMCID: PMC4383998. 3. Li, Tong, et al. "Cysteinyl‐tRNA formation: the last...difficulties with kinase specificity in vitro. The establishment of a system in which purified, recombinant protein...interested in the intersection of cell signaling, metabolism, and the mighty mitochondrion. You can follow...

...common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple...infected cells, an idea that became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM...enough T-cells could be reached to ablate a well-established viral reservoir. A second challenge for CRISPR...developing resistance. Shortly after Kaminski et al. published their results, Wang et al. showed that HIV-1 could...

...343-345. PubMed PMID: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9...

...22205518. PubMed Central PMCID: PMC3574576. Lai, Sen-Li & Tzumin Lee. “Genetic mosaic with dual binary transcription...multiple genes at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not ...doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., Lisa K. Comstock & Mark Vasser. “The tac promoter: a ...

...Cordell JL, Cragg MS, Šerbec VČ, Jones M, Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa...unless you share the results with others. If you publish data generated using your antibody, it’s best scientific...

... . https://doi.org/10.1038/mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard...Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio...Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large...

.... Biol., Apr. 2016. PubMed PMID: 27075976. 4.  X. Li, E. R. Burnight, A. L. Cooney, N. Malani, T. Brady...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...team.     Mathew Goodwin is a Technical Sales Specialist at Transposagen. As a gene editing enthusiast...Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent Stem Cells:... Perry, V. V. A. Steffey, K. Miller, and D. W. Allison, “Decoding the Epigenetic Heterogeneity of Human...

...science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton JM, ... treat disease postnatally. Now, three papers published concurrently in Science have shown CRISPR can ...

... N., Ma, W., Liu, X., Cai, Y., Kou, J., Wang, J., Li, L., Peng, S., Xu, Z., Zhang, W., Qiu, Y., Wu, Y....the first paper identifying a binding receptor published this year (Dhungel et al., 2025)! It can transduce...contributing their expertise! This post was originally published in January 2025 and updated in August 2025. References...

... PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. and Court, D. L. 2014. Recombineering...Central PMCID: PMC307639. 2. Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland...

... than APC. While APC's global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum...

...plasmids from this paper at Addgene. 6. Xing, Hui-Li, et al. “A CRISPR/Cas9 toolkit for multiplex genome...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...