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  1. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...summer months, but this is also our best month ever! Hats off to all of our wonderful writers and all those...Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry & Small Molecule...

  2. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest (blue) in...is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction occurs which...

  3. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...terminal repeat (ITR) sequence Inverted terminal repeats (ITRs) are what makes an AAV transfer plasmid an...(2019) A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Human Gene Therapy Methods...

  5. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with research! It...

  6. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...this promoter-gene combo are two long terminal repeats (LTRs). These LTRs define the limit of what will...constructs. Sandwiched between two inverted terminal repeats (ITRs) is the promoter and cargo, in that order...

  7. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...informal settings like lab meetings, Twitter, coffee chats with potential collaborators, blogging and more....more. These each have different goals, formats, and processes, and they provide different amounts of access...

  8. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...viral handling. Gloves are always required and lab coats are either recommended or required in BSL-1 and ...require or recommend disposable gowns over your lab coats and double gloving, so that, if needed, a layer ...

  9. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...Monomeric StayGold variants By Mike Lacy In 2022, Atsushi Miyawaki and colleagues identified a highly bright...endothelial cells across diverse mice strains and rats. Figure from Chen et al (2023) under CC-BY license...

  11. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka et al., 2023), but these effects were very small...5367–5372. https://doi.org/10.1021/nl202950h.  Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto...

  12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish between models for trafficking....2 (2009): 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. ...

  13. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...persistent transgene expression in certain cell types (Tatsis et al., 2007), potentially because some of the ...doi.org/10.1038/s41586-023-05949-1. PMID: 36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy...

  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... Can be distributed in digital or analog media formats. Cognitively demanding format. Equipment, expense...incorporate visuals if needed. Traditional media formats are quite competitive while digital can be oversaturated...

  15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...causes a shift towards antidepressant behavior in rats in the forced swim test as seen by increasing the...hippocampus evokes antidepressants-like effects in rats." Psychoneuroendocrinology 46 (2014): 78-87. PubMed...

  16. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...second-order structure of the AAV genome, caused by repeats and self-complementarity of the AAV genome and ...binding sites between the primers and the AAV genome repeats. Primer annealing can be affected by the presence...

  17. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ..., a pooled library of scFvs is displayed on the coats of bacteriophages, a process which links genotype...pooled library of scFvs that are displayed on the coats of bacteriophages. This library is incubating with...

  18. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...indeed the culprit in egg white injury by feeding rats purified avidin (Kresege et al., 2004). Avidin is...de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation...

  19. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...do identify as Democrats more often than Republicans, the March is not about Democrats or Republicans ...

  20. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...large inserts (>7 kb)(Berg et al., 1989), direct repeats (Ribeiro et al., 2009), and at high copy numbers...plasmid recombination mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007...
Showing: 21 - 40 of 155 results