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We narrowed to 79 results for: Blue fluorescent Protein

Showing: 21 - 40 of 79 results

  1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ... awards to early career scientists using fluorescent proteins in their research. These awards help early... in the development and dissemination of fluorescent protein tools. This year, we’ve made some changes...inhibits the neuron (via hM4D). She fused fluorescent protein tags to the receptors to monitor their localization...are co-expressed with a photoconvertible fluorescent protein mEOS4a. Cells containing a sensor that shows...-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling...disease,” says Aggarwal. This biosensor changes fluorescence based on the release of glutamate from neurons...thiol-containing acceptors. These hydropersulfides modulate protein activity and have important roles during oxidative...

  2. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...interested in our fluorescent protein background. Download the orange fluorescent protein background.     ...not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and ...out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can find all of the...with another DNA background option, Download the blue DNA background. Or get the same background as a ...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators (GEVI...sensor and a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness ... Examples of some genes encoding gap junction proteins cx43 GJB1 GJB2 Bioelectric states can alter ... even between many cells (through gap junction proteins which connect the cytoplasm of two cells) can ...controlled by the opening and closing of ion channel proteins, they are not always easy to track at the transcriptional...Arch (green-light activated proton pump) and ChR2 (blue-light activated cation channel) light-activated ...anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization...

  4. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... 2015). To do so, they fused a different fluorescent protein to various Cas9 variants, SpCas9, NmCas9,... or boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of...MS2-PP7) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to visualize relative...blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR blog posts Resources...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...identical hairpins within one gRNA will result in a blue, green, or red readout. But gRNAs containing two...boxB sequences inserted into the sgRNA generates blue, green, or red colors for labeling. A combination...

  5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...when paired with appropriate fluorescent optics. Existing red fluorescent voltage indicators, however,...inhibitory rhodopsin (Ace2N) with the red fluorescent protein (mScarlet) with either a seven or eight amino...manipulations when combined with existing green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find...zebrabow. In this system, cells express various fluorescent proteins controlled by Cre recombination. Each cell...of colors depending on the combination of fluorescent proteins expressed. CreLite-mediated recombination...editors that can be reconstituted in vivo to form a protein identical in sequence to the unmodified enzyme....31937940. Detection of neural activity using a red fluorescent genetically encoded voltage indicator Article...

  6. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...Photoactivatable Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with... other kinases and proteins. Zhou et al suggest that more photoswitchable proteins could potentially be... reporter plasmids Find photoactivatable fluorescent proteins in empty backbones in the Michael Davidson...Photoswitchable Fluorescent Proteins ... fluorescent proteins...transduction pathways are a lot like cell phone networks. Protein kinases deliver messages to the next members of... the active site. Dronpa also produces green fluorescence when exposed to 500 nm light.  Under 400 nm ...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...indicators (GECIs) are fluorescent proteins linked to calcium binding proteins (e.g., calmodulin or troponin...methods Many calcium reporters use a single fluorescent protein (FP), requiring only a simple measurement.... This sensor switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled...Structure-and mechanism-guided design of single fluorescent protein-based biosensors. Nature chemical biology...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services  ...troponin C). Their fluorescent readouts are used to monitor calcium concentrations and transients in living... of the sensor, and (3) subsequent change in fluorescence only occurs at high calcium concentrations. ...

  8. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ... a given protein is to fuse it to a fluorescent protein. You can then study where the protein is localized... Sign Up to Receive the Fluorescent Protein eBook Avoid Fluorescent Protein Oligomerization Learn about... Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase... the brightness in your fluorescent imaging experiments. Fluorescent protein fusions One of the easiest...problems? Instead of directly fusing a fluorescent protein to your protein of interest, you instead fuse it ...on a protein-by-protein basis. However, since many of Addgene’s SunTag plasmids have coveted “blue flames...overexpress your protein at higher levels, you risk observing artifacts only present when the protein is at a ...

  9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...many fluorescent proteins. Additional fluorescent protein tools Photoactivable fluorescent proteins (proteins...Laser include: Blue-green fluorescent proteins like EGFP Yellow-orange fluorescent proteins like TagRFP,...Choosing Your Fluorescent Protein Learn Some of the Pitfalls of Working With Fluorescent Proteins Use FRET ...Monitor Protein-Protein Interactions Resources on Addgene.org Check out Our Fluorescent Protein Guide ...the visualization of fluorescent proteins expressed in the specimen.  2. Fluorescence Activated Cell Sorter...using cells labelled by fluorescent chemicals or by other fluorescent proteins, labs were also able to...that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins (protein that...

  10. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...al. A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods 12, 763... Medicine. Several sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created...Addgene. Biliverdin chromophore Near-infrared fluorescent proteins (NIR FPs) were derived from natural photoreceptors... about cellular events via  fluorescence changes occurring due to protein synthesis, degradation, or reconstitution...Shcherbakova et al., 2018). For detection of protein-protein interactions, we recommend using NIR split ...expertise is development and applications of fluorescent proteins, genetically encoded biosensors, and optogenetic... to Increase Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells. Cell Chem Biol 24, 758...

  11. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...Choosing Your Fluorescent Proteins for Multi-Color Imaging Choose the Brightest Fluorescent Protein for Your...Addgene.org Browse Our Fluorescent Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids...super-resolution (3). Fluorescence microscopy techniques   Fluorescent Widefield Microscopy Fluorescent Widefield...block out of focus fluorescence from reaching a detector or restricting fluorescence to specific excitation... of blue to excite a molecule of GFP. Because red light has approximately half the energy of blue light... fluorescent proteins... into noise. Due to its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...cleaves the supplied fluorescently labelled ssDNA substrates. Upon cleavage, the fluorescence is no longer quenched...resulting fluorescence can be observed under either no excitation light, UV light or LED blue light.  Highlights... cleavage of fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that... 9V battery which uses a Blue LED and an orange filter to detect fluorescence upon cleavage. Highlights...streptavidin portion of the probe via biotin. The fluorescent portion of the probe that is cut will continue...and released when cleaved. Cleavage results in fluorescence and this approach can be incorporated into a...90 minutes Equipment needed: 37°C heat block, LED blue light illuminator or UV light illuminator. Approach...

  13. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection...Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael...searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection webpage. These plasmids are divided... tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor labeling and... plasmid backbones spanning a rainbow of fluorescent proteins “It’s a great collection with over 300 backbones... impressively big deposit. “He has every fluorescent protein under the sun, from the standard oldies but...interest isn’t in the ORF collection, the fluorescent proteins are, which makes mixing and matching a relatively...

  14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...b(right)est fluorescent proteinFluorescent Proteins 101: A History of Fluorescent Proteins  ...stability of fluorescence over time is crucial. For instance, the fluorescence of fluorescein isothiocyanate...principles of fluorescent dyes/ligands Figure 1: Example structures of fluorophores, fluorescent dyes,...dyes, and fluorescent ligands. Figure created with biorender.com. Fluorescence refers to the emission...attached to self-labeling proteins. Some common examples of these self-labeling proteins would be SNAP-tag® ...through fluorescent labeling. There are several advantages to using these self-labeling proteins over traditional... traditional fluorescent protein tags, such as higher specificity, increased versatility in probe attachment...

  15. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...inside of your cells. While proteins themselves can emit intrinsic fluorescence when excited with ultraviolet...amino acids found in all proteins, so you can't distinguish the different proteins from each other. To distinguish... A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or mCherry...through detecting the reporter's fluorescence. Another way to label proteins is through treating cells with...bright" for the blue laser. To understand why that is, let's look at Figure 2. The blue laser excites at...distinguish different target proteins in your flow analysis, you'll need to use fluorophores that attach...mCherry, through genetic modification. As your protein of interest will only occur in combination with that...

  16. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to ... a split fluorescent protein (FP) and measure the signal produced when the candidate proteins’ interaction...assemble the full structure of the fluorescent protein, producing fluorescent signal. Image modified from Feng...Multiplexed labeling of cellular proteins with split fluorescent protein tags. Tamura R, Jiang F, Xie J,...Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis... and GFP(11) Versatile protein tagging in cells with split fluorescent protein. Kamiyama et al. Nat Commun...

  17. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...VVDfast homodimerizes under blue light iLID-SspB heterodimerizes under blue light BcLOV4 undergoes a conformational...plasma membrane under blue light CRY2-CIB1 heterodimerizes or forms clusters under blue light Check out our... or CIB1 to your protein of interest and express the other separately, then use blue light to generate... implant or a worm wiggling back and forth under blue light — but there’s a lot more to optogenetics than...photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...SspB-mCherry-p60 (katanin) are recruited to microtubules under blue light, removing tubulin dimers from the microtubule...license. A naturally occurring microtubule-severing protein, Katanin is of course named for the samurai sword...

  18. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ... found that fluorescent proteins might not always be the best option. Fluorescent proteins can interfere...that non-fluorescent tags may be advantageous over fluorescent proteins when the fluorescence is not essential...fused to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most widely used ...green and red fluorescent proteins) allow simultaneous identification of more than one protein of interest...interfere with fused protein function Under some circumstances, the fused fluorescent proteins can be a roadblock... in the expression of the protein of interest. The green fluorescent protein (GFP) and mCherry, two of...trafficking of the fusion protein (Kimple et al., 2013). We found that the fluorescent proteins can, indeed, interfere...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...where an antibody binds a target protein in your sample, then a fluorescently labeled secondary antibody binds...suitable antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based...reagents like fluorescently-labeled nanobodies or scFvs. Many, many options for fluorescently conjugated ... palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show...Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence Resources...then you use a microscope to see where the target protein is (localization) and how much there is (quantification...quantification). But what if you’ve got several interesting protein targets and you want to know if they co-localize...

  20. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...stranded DNA bound by tTALE-FP with the fluorescent protein fluorescing green. Image from Shin et al., 2019...intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein...DNA. TALE proteins can bind specific DNA sequences which brings the attached fluorescent protein to the ...10.15698%2Fmic2020.04.713 Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed...contributed by Leah Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular... in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent...this vector is suitable for flow cytometry and fluorescence microscopy, they also show that this method ...
Showing: 21 - 40 of 79 results