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We narrowed to 208 results for: CIL

Showing: 21 - 40 of 208 results

  1. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...results you get back from your favorite sequencing facility. When it comes to DNA sequencing the chromatogram...your sample or consider asking your sequencing facility to apply a special protocol for a difficult template...you send for DNA sequencing If your sequencing facility requires you to perform your own Big Dye PCR amplification...

  2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...devised means of co-opting phage genetic systems to facilitate homologous recombination in bacteria. These quirks...of just a single organism, such as Clostridium difficile, could be targeted and preferentially depleted...system could be further refined by the addition of a uracil DNA glycosylase inhibitor and degradation tag on...DG (2020) In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials...

  3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...contains an ampicillin resistance marker in the backbone. A selection on agar containing ampicillin will yield...on this below). Plating the transformation on ampicillin plates containing X-gal allows you to identify...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...efficient mechanism of homologous recombination to facilitate simple gene replacement/mutation. Yeast expression...L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine (uracil) yes - Grow with 5-FOA. S. cerevisiae yes - This...are used in the growth media. ura4+ pyrimidine (uracil) yes - Grow with 5-FOA*. S. pombe  no no *FOA in...

  5. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...strains are ∆ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes... isolate transformed strains on media that lack uracil, but contain methionine. (This allows cells carrying...Call to Action, Washington, DC. 2. President's Council of Advisors on Science and Technology [PCAST] (...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...Addgene’s repository. For long-term storage in our facilities, every sample is transferred to a 2D barcoded...Pir1. The majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along...kept at Addgene and another is sent to a storage facility). The third tube is used to fulfill orders. Breaking...

  7. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...performed by the facility that produced the viral vector lot. Contact us to determine which facility produced ...collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania Vector...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...with the Cas13a molecule through a uracil-rich stem loop and facilitates target binding and cleavage through...system, Cas9 uses both a tracrRNA and crRNA to facilitate binding and cleavage of target DNA respectively...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... lab harnessed a group of bacterial toxins to facilitate efficient recombineering (Khetrapal et al., 2015...reduce transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation...system (Lazarus et al., 2019). Additionally, to facilitate modification of strains where conjugation may...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...and a transposase. The piggyBac™ transposase facilitates the integration of the transposon specifically...achieved using the piggyBac™ transposase, which facilitates direct integration of the donor-transposon (carrying...standard homology directed repair template to facilitate the selection of cells containing your desired...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...develop better strategies for combating cancers. To facilitate innovative cancer research, the Reference Reagents...express the integrated gene. These vectors contain uracil-excision based (USER) sites, flanked by ADH1 and...by the Church lab, termed “dCas9-VPR”, which facilitates robust activation of target genes (20-40x greater...compensating for histidine (HIS3), leucine (LEU2), uracil (URA3), methionine (MET17), and lysine (LYS2) deficiencies...

  12. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C...

  13. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...yourself time if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to...transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’ve found that...

  14. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... Protein Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes...other than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved...

  15. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...

  17. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...

  18. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...apart; however, they still remain attached to the ciliate. It’s thought that they function as a kind of armor...Defensive extrusive ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are...

  19. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...develop the web page. The team manager must then facilitate appropriate communication between members of ...only way and may not even be the best way. This facilitates success and trust. Micromanaging is bad for everyone...

  20. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...antibody-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing Monoclonal...doi.org/10.1074/jbc.m116.736942  National Research Council (US) Committee on Methods of Producing Monoclonal...
Showing: 21 - 40 of 208 results