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We narrowed to 361 results for: ERG

Showing: 21 - 40 of 361 results

  1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...after undergrad] was to learn and speak the language of science. I didn’t come out of [undergrad] feeling...solidified some concepts that are heard often during undergrad. Lauren Flynn is the Addgenie behind our...doctor. After taking a year break in between undergraduate school and studying for the MCAT, she decided...description.” Our resident bagel provider, source of energy, and conversation master knows that although her...

  2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...science.   Acknowledgements Chan Zuckerberg Initiative Chan Zuckerberg Biohub Manuel Leonetti Ryan Leenay...Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We hereby announce the general availability... us your questions or comments to gdingle@chanzuckerberg.com. We're eager to hear your thoughts. Many...Greg Dingle is a software engineer with the Chan Zuckerberg Initiative, working on assignment at the Biohub...

  3. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...Cressey is a PhD Student in the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School...Medicine, Department of Microbiology, National Emerging Infectious Disease Laboratories.  She is interested...The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166. 3. Hoenen,...21699921. PubMed Central PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription... 9971816. PubMed Central PMCID: PMC104478. 5. Mühlberger, Elke. "Filovirus replication and transcription...

  4. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...skills, but I’ve learned a lot since my days as an undergrad working with cancer cell lines. As I started planning...useful tasks. The quick accomplishments will likely energize you. For example, let's say you’re doing a multi-day...that should help keep you content and give you the energy to finish any additional work you might need to... all if I’d planned appropriately. Back in my undergrad days, I had general goals for my experiments, ...to it each day. Once you get over the activation energy barrier that comes with making ANY progress, the...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...proteins (GPC1, GPC3, and GPC4). All IPI antibodies undergo extensive characterization at IPI as well as community-led...CRISPR homing of retroelements." The Abudayyeh-Gootenberg Lab developed this tool based on several new ...Lim, J., Hiraizumi, M., … Abudayyeh, O. O., & Gootenberg, J. S. (2025). Reprogramming site-specific retrotransposon... The enhancers span all four major classes of GABAergic interneurons — those marked by parvalbumin (PV...lysosomal-associated membrane protein 5 (LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The...

  6. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...intracellular domain of an endogenous GPCR, such as adrenergic receptors. When exposed to light, there is a ...Figure 3: Chimeric OptoXR incorporating rhodopsin undergoes a conformational change when exposed to blue-light.../978-1-0716-0755-8_2 Boyden, E. S., Zhang, F., Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale..., N., Berthold, P., Ollig, D., Hegemann, P., & Bamberg, E. (2003). Channelrhodopsin-2, a directly light-gated...Dieter, A., Pieper, F., Sims, R., Tenedini, F., Bergs, A. C. F., Bendifallah, I., Zhou, F., Zeitzschel...

  7. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...archaeal immune systems has led to extreme sequence divergence that requires a multi-pronged analysis to sort...References and Resources References Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J...doi.org/10.1126/science.1231143 Cox, D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner...Makarova, K. S., Koonin, E. V., Abudayyeh, O. O., & Gootenberg, J. S. (2021). Programmable RNA targeting with...nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, A., & Marraffini, L. A. ...

  8. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...Bioluminescence Resonance Energy Transfer (BRET). BRET is similar to Förster Resonance Energy Transfer (FRET), ...fluorophore. The difference for BRET is that the energy transfer is between a luminescent donor and a fluorescent...protein dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the drug,...

  9. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was collected...expression changes of more than 100,000 cells undergoing iEP conversion, the Morris Lab found that cells... to uncover two distinct trajectories of MEFs undergoing iEP conversion, but CellTag could be used to ...study other biological processes where cells are undergoing identity changes, such as the differentiation...

  10. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...transposons, the Sternberg and Zhang labs developed CRISPR-transposons. The Sternberg lab's system called...and Gram-positive bacteria Jason Peters, Oren Rosenberg, and Carol Gross used two different genetic systems...References Bikard D, Euler CW, Jiang W, Nussenzweig PM, Goldberg GW, Duportet X, Fischetti VA, Marraffini LA (2014..., Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS (2019) Enabling genetic analysis of diverse...

  11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...suddenly cool: DNA methylation. In 1983, Andrew Feinberg and Bert Vogelstein showed, for the first time...methylation detected in a human disease: cancer (Feinberg & Vogelstein 1983). The floodgates, as they say...methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization, this method...827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A, Vogelstein B (1983) Hypomethylation distinguishes...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...Health Organization has declared a global health emergency and called for the fast-tracked development of... was underscored by the potential scale of the emerging outbreak, as many as 2.2 billion people globally...prototyping and sensor assembly pipeline for future emerging pathogens or health crises. Many thanks to our..., and self-assembly for applications in health, energy, and sustainability.   References 1. Pardee, Keith...

  13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ... visible and infrared light to dissipate excess energy at wavelengths of low photosynthetic activity. ...lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure 4)...10.1074/jbc.m606921200 Dove SG, Takabayashi M, Hoegh-Guldberg O (1995) Isolation and Partial Characterization...48604-4 Salih A, Larkum A, Cox G, Kühl M, Hoegh-Guldberg O (2000) Fluorescent pigments in corals are photoprotective...

  14. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...fluorescent proteins (Gambarotto, et al. 2019; Tillberg, et al. 2016). That being said, the anchoring,.../doi.org/10.1371/journal.pbio.3001020  Chen F, Tillberg PW, Boyden ES (2015) Expansion microscopy. Science...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...101796. https://doi.org/10.1016/j.mex.2022.101796  Tillberg PW, Chen F, Piatkevich KD, et al (2016) Protein-retention...

  15. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...real goal. We are all constantly negotiating our energy and attention. The goal is to maximise the time...Check out Joanne's Reddit AMA Managing time and energy between work and the rest of life is sometimes ...will worry less about how they will handle an emergency and this will make you much more confident about...daughter vehemently reminded her doctor that she was allergic to penicillin – I am much less worried knowing...

  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...specific neuronal subtypes such as inhibitory GABA-ergic interneurons, Gordon Fishell’s lab has developed... you to target and functionally manipulate GABA-ergic interneurons across species and will undoubtedly...You can find the AAV vectors for infecting GABA-ergic interneurons here. Dimidschstein J, et al. Nature... use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging...Under the supervision of Dr. Tan, a team of undergraduates created DNA ladders (aka DNA molecular weight...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...and expand our current knowledge. The Abudayyeh-Gootenberg lab (also called the AbuGoot lab), a joint lab...lab run by Omar Abudayyeh, PhD, and Jonathan Gootenberg, PhD, focuses exclusively on this space — developing...Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable RNA targeting with the single-protein...Adrianna E. Zepeda, Kevin Holden, Jonathan S. Gootenberg, Omar O. Abudayyeh. Drag-and-drop genome insertion...

  18. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...update of this post. References Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto...://doi.org/10.1186/s13059-018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung...nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...://doi.org/10.1126/science.aba8853 Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, ...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...: 797459. Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322...frequently in plasmids with large inserts (>7 kb)(Berg et al., 1989), direct repeats (Ribeiro et al., 2009...and define recA-independent mechanisms. Avoid overgrowing the bacterial culture Another way to prevent ...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light at ...release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally ...FRET signal. FRET is a non-radiative transfer of energy from one fluorescent protein to a compatible fluorescent...other fluorescent probes but can still transfer energy to the fluorescent protein acceptor. NLuc is an...
Showing: 21 - 40 of 361 results