Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 303 results for: GAI

Showing: 21 - 40 of 303 results

  1. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...use two antibodies - one against your target of interest and a second against the first antibody. When.... (C) A third solution is to use Fab fragments against the sample species to block the binding sites on...

  2. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...and find an ideal antibody against Protein A and an ideal antibody against Protein B. The antibodies are...target and can be used with secondary antibodies against the new Fc region. In addition to increasing multiplexing...

  3. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...said, “Science is failing over and over and over again until you don’t.” As every researcher can attest...- within that process of failing, over and over again until you don't, of trying to understand your completely...understanding why your experiment has not worked…again…for the seventh time….   I find that once I sit ...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...Paris and New York. We want YOU to join the fight against coronavirus We are just at the beginning of understanding...next scientist joining in this worldwide fight against SARS-CoV-2. As we’ve all been affected in various... UCSF (the QCRG network coming to the rescue -- again!). Also, we’ve got to mention that our FedEx guy...

  5. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... Although it’s possible to generate antibodies against many types of molecules including proteins, lipids...hybridomas come from animals that were immunized against the target protein. Animals are first injected ...special medium called HAT. This medium selects against unfused myeloma cells and antibody-producing B ...

  6. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University have developed...signal. Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored...imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing...

  8. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...it can also be used to characterize antibodies against the antigen bound to the plate. Figure 3...absorbed by a solution - of these wells is plotted against the known protein concentration. Importantly, you...: A standard curve is generated by plotting OD against known concentrations of your target protein. The...

  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...other hand, vectors derived from AAV have recently gained popularity as they are uniquely suited gene delivery...landmark study showed significant clinical efficacy against a form of blindness known as Leber’s congenital...example, prevalence of neutralizing antibodies against some AAV types, AAV1, 2, 3 and 5 are thought to...

  10. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...yourself a bit of a break before you read over it again with fresh eyes and correct it (and, believe me,...and I have certainly found that the experience I gained in writing small travel grants to attend conferences...second) you won’t succeed, but you must try, try again. Now, this does not mean that you should apply for...

  11. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...50% per freeze thaw cycle (Krajden et al., 1999;  Ugai et al., 2002). If the virus will be used in a couple...of ensuring viral production is to perform PCR against components of the viral vector using the harvested...PMID: 10325307. PubMed Central PMCID: PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...probably never use the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and to repurpose...

  13. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...spreads through the wild population and immunizes it against the first drive. Laboratories interested in building...question), why not use them whenever applicable?Against this, consider the cost of an accidental release...accidental release could delay real-world applications against scourges such as malaria and dengue for many years...

  14. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...purification with glutathione, can also protect against proteolysis. That’s one form of instability. Prokaryotes...Good for purification with glutathione; protects against proteolysis, but may reduce solubililty HA YPYDVPDYA...  Combo See text  TRX MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL...

  15. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...opportunities. Many thanks for our guest blogger, Gairik Sachdeva! Gairik Sachdeva is a Ph.D. in Bioengineering and...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...gut-wrenching, but you can get that feeling of camaraderie again in a non-academic career. You might not be in a ...you still find the idea of not holding a pipette again upsetting, there are ways to get back to the bench...opportunities. You can get your hands in nitrile gloves again! The opportunities are there once you start looking...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Brian Kaspar and Steven Gray) and two MECP2 labs (Gail Mandel and Stuart Cobb) to create the Gene Therapy...for precise correction of Rett syndrome mutations. Gail Mandel and her team have shown for the first time...being pursued at Vico Therapeutics, co-founded by Gail Mandel. There are numerous technical challenges ...

  18. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...every experiment, an investigator needs a standard against which the results can be compared; results from...single experiment wherein Plasmid A (encoding shRNA against human Gene X in backbone Y) was transfected into...included as these provide the baseline/standard against which other samples can be compared. The Empty ...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...secondary antibody that recognizes the primary, wash again, then image. But here’s where the “sequential” part...add the new primary, wash, add secondary, image again, inactivate, and repeat these cycles for all your...yourself. If you can’t find a suitable antibody against your target protein, consider fusing a protein ...

  20. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...instead of the population as a whole, scientists gain statistical power in their observations. However...receptor are stained with a fluorescent antibody against the receptor and analyzed on a flow cytometer. ...methods. In direct staining, the primary antibody against a target is conjugated to a fluorochrome. In indirect...
Showing: 21 - 40 of 303 results