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We narrowed to 102 results for: Gene Tools Blue

Showing: 21 - 40 of 102 results

  1. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...sequencing of plasmids isolated from blue or white colonies. Sequencing of blue colonies showed the expected ...result in a blue phenotype. Indeed, this was seen in all lacI/ lacZ assembly test systems; no blue colonies...in optimization of assembly systems) to produce a blue colony phenotype upon growth on LB/Cam/X-gal/IPTG...expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a mixture of... of assembly DNA assembly methods are important tools for many areas of science, and researchers continue...PMCID: PMC6061786. Additional resources on the Addgene blog Learn the basics of Golden Gate assembly ...other plasmid cloning techniques Resources on Addgene.org Find basic molecular biology protocols Find ...

  2. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools to use,...information on hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide a powerful... useful tool for neuroscientists! Since then, scientists have developed many optogenetic tools to control...cells expressing the optogenetic tool causing a change in cellular activity or gene expression. Experimental...BioRender.com.   Optogenetic tools Types of control We will discuss three types of tools: light-gated ion...researchers flexibility in using optogenetic tools in their experiments. Some tools turn on and off rapidly with...considerations for using optogenetic tools Once you’ve selected the class of tool appropriate for your experiment...

  3. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced Table 1: Summary...imaged while cycling between dark and blue light which revealed a blue light-dependent shift in mCherry fluorescent...insertion caused blue-light induced binding. The lab used this general design scheme to generate two more OptoNBs...always generated OptoNBs with dark-induced binding, but insertion into loop 6 generated both blue light...with OptoNB coated beads and wrap the column with blue LED lights. When you switch off the lights, OptoNBs...proteins flow through. To elute YFP, you turn on the blue lights. Which option do you choose?  Option #2 ...whose binding of their target protein is altered by blue light illumination. This ability to reversibly bind...

  4. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...researchers to make innovative tools not possible with other luciferases. In these tools, NanoLuc-fluorescent protein... be paired with optogenetic regulators. Many of these tools are available from Addgene and we're excited...repository. We encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science better when... bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc...Enhanced Nano-Lanterns. These great imaging tools generate cyan, yellow, green, orange and red fluorescence...release with an optogenetic receptor, melanopsin, requiring brief exposure to 470nm blue light. To avoid...avoid overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...

  5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...Eisenhoffer lab, is a new tool that increases temporal and spatial precision of gene expression and reduces...limits the delivery of larger transgenes, such as base editors which have a gene size of ~5.2 kb. To overcome...spectrally separate from green sensors and blue-light-activated optogenetic actuators.  To overcome this overlap...from the MoClo Toolkit from Sylvestre Marillonnet’s lab to develop synthetic biology tools for cyanobacteria.... Light up CreLite: a red-light inducible optogenetic tool for precision in cell lineage tracing Article...the breadth and versatility of this tool. Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101...Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were...other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green 499 - 519...Specifically, these proteins are excited by UV light or blue light and their emission is green or red light. ...-FPs. If you do not have a filter that will pass blue light to the detector/camera, then BFPs are of no...you're looking for more fluorescence microscopy tools and techniques to aid your work, head over to greenfluorescentblog...Medicine. He is interested in everything related to gene expression, particularly at the RNA level. He maintains...fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics” ...

  7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... With no blue light, the channelrhodopsin remains inactive and neurons don't fire. With blue light, ions...delivery enables minimally-invasive gene delivery and optogenetic excitation. Excitation of neurons deeper...which is needed since current channelrhodopsins tools have low conductance, but also confines channelrhodopsin... injection into mice. A fiber-optic cable allows blue light to reach the brain. Created with BioRender.com...the differences between chemogenetics and optogenetics Resources on Addgene.org Browse all viral vectors...available at Addgene Visit our viral vectors guide Find our chemogenetics and optogenetics science guides...resources on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about...

  8. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...development and dissemination of fluorescent protein tools. This year, we’ve made some changes. In addition...the dual regulation, she could then use pulses of blue light to activate the neurons at specific firing...(B) CR-Cre mice demonstrate pain response due to blue light stimulation of dual virus-expressing CR neurons...painful responses in the left and right paw due to blue light activation and CNO silencing. (D) Immunohistochemistry...developed a dual virus system using both optogenetics and chemogenetics to study the activity of parvalbumin...in the lab. He has recently developed a third-generation intensiometric glutamate sensing fluorescent ...catalyzes the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred...

  9. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord... the Addgene Blog Important considerations when using AAVs Learn more about using AAV as a tool for gene... central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate...organs to the CNS, are also an important target for gene delivery, especially for the study of pain. While... two new AAV capsids and how they can be useful tools for studying the CNS and PNS! What is the CREATE...all possible combinations into a region of the cap gene. In this special CREATE cap plasmid, loxP sites ...primers to determine which version(s) of the capsid gene successfully infected the cell type of interest....

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome... researchers with many different ways to control gene expression. Get the full scoop from Addgenie A. ...express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants...how Cas9 homolog, Cpf1, may make it easier to make gene edits at new genomic locations. 6. CRISPR 101: Non-homologous...Adeno-associated viral (AAV) vectors are useful for in vivo gene delivery, but have limited packaging capacity making...2015 has been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array ...have started providing recorded interviews with Addgene community members. In addition we’ve experienced...

  11. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...and greatest plasmid tools! Thank you depositors! In fact, this year, Addgene depositors quickly stepped...featuring the work of the Addgene community. Perhaps you’ll find plasmids and new tools that will speed your...deposit? Thursday: Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve... 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500...whether you are a depositor or not. We will have Addgene trivia (with prizes!), polls, and shout outs, and...We’ll also take a look back on some fascinating Addgene history that you might not have known about. Here's...

  12. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...conventional optogenetic tools." In total, the researchers applied the LITE system to 28 different genes to establish...designed to bind specific genes and turn them on or off, literally at the flip of a (blue) light switch. Zhang...functions, Konermann needed tools to manipulate the expression of specific genes in the brain in reversible... enable. In a Nature paper introducing the optogenetic tool, Konermann and Zhang showed they could package... after light stimulation. Under those optogenetic controls, gene expression levels reliably increased ...factor of 10 to 20. A flexible and modular optogenetics tool The researchers also showed just how flexible...could be by applying it to altering gene expression via epigenetics with the addition of histone modifiers...

  13. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...Vedantam CRISPR/Cas9 is widely used genome editing tool. However, Cas9’s endonuclease activity can be limited... role in the transcriptional regulation of many genes. A number of groups have designed means of studying...Arabidoposis protein that heterodimerizes with CIBN upon blue light exposure (see Fig 1*). When the group targeted...as increased Zfp462 expression within 4 hours of blue light induction (6-fold faster than previously developed...Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed...MerMAIDs - marine channelrhodopsins for the optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting...plasmid deposit please sign up here.        New toolkit for N or C terminal tagging of proteins for degradation...

  14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... in popular FPs. This generated a palette of inert green, cyan, yellow, and blue variants that can be ...FPs have been undeniably powerful and successful tools that have enabled numerous cell biology assays. ...proteins of interest with a combination of green and blue or cyan and yellow moxFPs. The inherent brightness...negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using CRISPR ...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila... et al. "Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the...quantitative imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing...

  15. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...and a filter set, many filter set vendors provide tools to plot the fluorescence spectra of proteins and...shown in the lighter colors; excitation spectra are blue and emission spectra are red. Neither is a perfect... tag your least abundant proteins, with the blue and infrared channels used for more abundant proteins...TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will ...set of proteins the green and red proteins are generally the most detectable and so should be used to tag...

  16. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... design tools Many of the above-listed plasmid mapping tools have built-in primer design tools, but here...protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs, aligns...optimization tool: To access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence...plasmid analysis tool including editing, annotating, creating maps, and more. This tool is maintained by...Sequence tool.   DNA sequence manipulation tools  Sometimes DNA sequences need to be “cleaned up” or ... analyze them. These tools help with that.  DNA sequence reverse complement tool: Enter a DNA sequence...bioinformatics tools and knowledge databases. Do you have a favorite online molecular biology tools not listed...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the ...demethylation Optogenetic repressors of gene expression in yeast Megan McClean’s lab generated an optogenetic... available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth...help you find more new CRISPR tools for your research! This time: Optogenetic repression in yeast Base editing...optogenetic toolkit that controls repression of gene expression in Saccharomyces cerevisiae. The system consists...2019. Fusing epigenetic modifiers such as TET1 with inactive Cas enzymes can alter gene expression via...plasmids If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included...

  18. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...multiplexing, GECO sensors are available in green, blue, red, and far-red. There is also a ratiometric GECO...measurements with certain single FPs (such as GEX or blue/green CEPIA) or through FRET sensors, can reduce...viral vectors have to do with all of these cool tools? Calcium sensors are often delivered using viral...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...calcium sensors. Selecting a calcium sensor Genetically encoded calcium indicators (GECIs) are fluorescent...neuronal cell body or the axon (GCaMP6 only). The genetic elements required for Drosophila studies have been...chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to probe complex brain...

  19. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into the brains...for an adaptable tool that could continuously monitor multiple systems. Ideally this tool would be wireless... on the Addgene blog Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors...drug administration, and optogenetics. Enter neural fibers! The neural fiber tool  The neural fibers developed...making it difficult to probe due to the lack of tools to continuously monitor it. How to address this?... the intestines of mice. The result? A very cool tool to advance our knowledge of brain-visceral organ... The problem  Previously, there were very few tools available for monitoring how both the brain and ...

  20. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... is proving an exciting tool for producing specific genetic mutations and gene disruptions without the...both introduce genes into an organism’s genome (see figure) and to disrupt endogenous genes at the site ...of inverted repeats (green) flanking the gene of interest (blue) in a plasmid backbone. A separate plasmid...of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool kit allows the...first-generation transposase (as determined by transposition assays of antibiotic-resistance genes conducted...). In their Nature Genetics paper, the lab showed that efficiencies of stable gene transfer using SB100x... the rapid generation of stable transgenic cell lines which can robustly express your gene-of-interest...
Showing: 21 - 40 of 102 results