We narrowed to 84 results for: Gla
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TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....cloning tool was first reported over 25 years ago (Douglas and Howard, 1991, Bubeck et al., 1993). But why...jakefwatson and @JGarciaNafria. References 1. Jones, Douglas H., and Bruce H. Howard. "A rapid method for recombination...
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Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered a BFP variant...551:464–471 . https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....2016): 928-30. PubMed PMID: 27723752. 4. Fowler, Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring...Central PMCID: PMC4412028. 5. Araya, Carlos L., and Douglas M. Fowler. "Deep mutational scanning: assessing... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....Central PMCID: PMC3874208 Other Resources: New England Biolabs Gibson Assembly Overview Additional Resources...Protocols Gibson Assembly® is licensed to New England Biolabs by Synthetic Genomics, Inc. ... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....organizers wanted to touch on in organizer David Riglar’s blog post. Essentially the goal was to determine... Found Here Resources at Addgene Read David Riglar's FOR Boston 2015 Blog Post Read Our Career Blog... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....and can include: equipment, reagents, animals, glassware, and even people. When developing a budget, I ...reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I... -
Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....tuition fees had just gone up dramatically and, in England, you could not receive government loans to study... and use my other skills and interests. I was so glad when my future supervisors were keen for me to become... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m....Einstein and Bill Nye in their lab coats handling glassware with brightly colored hazardous liquids. Working...responsibilities can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....Out This Video to See How Green Listed Works at a Glance! CRISPR knockout screens: Full genome vs. custom...More information can be found through https://wermelinglab.com/. References 1. Panda, Sudeepta Kumar, et... -
Bringing Renewable Energy to Addgene
TypeBlog PostPublishedAug. 27, 2019, 1:08 p.m....from renewable energy projects right here in New England- the majority of which are solar, wind, or hydroelectric... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog PostPublishedDec. 20, 2016, 3:30 p.m....Plasmid Grant Read about Plasmid Grant Recipient, Gladys Kaba Resources on Addgene.org Check out our Microbiology... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog PostPublishedApril 5, 2016, 2:30 p.m....Seeding Labs Follow Seeding Labs on Twitter @SeedingLabs or on Facebook Additional Resources on the Addgene... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog PostPublishedDec. 16, 2014, 8:01 p.m....Viewed Posts on our blog from the past year. A quick glance shows that interest in CRISPR continues to grow... -
A Scientist's Guide to Ebola
TypeBlog PostPublishedOct. 16, 2014, 3:01 p.m....papers freely available here. -PLoS and The New England Journal of Medicine have similarly curated their... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog PostPublishedMarch 17, 2021, 2:40 p.m....Chicago Marathon to benefit Neurofibromatosis, New England. Additional resources on the Addgene blog Learn... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m.... green. In the late 1980s, another researcher, Douglas Prasher, got the idea that this new green glowing... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog PostPublishedMarch 7, 2019, 2:39 p.m.... scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use ... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog PostPublishedSept. 9, 2016, 2:43 p.m....What's in the CRISPR kinome library? Thus, we are glad to provide CRISPR libraries targeting the mouse ... -
Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....The Discipline of Teams by Jon R. Katzenbach and Douglas K. Smith or the book on which this article is based... -
Celebrating Accomplishments in the Lab
TypeBlog PostPublishedMay 8, 2014, 3:07 p.m....parties - despite BBQs being wishful thinking in England. The Cepko Lab throws going away parties and potlucks...