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We narrowed to 51 results for: HDR

Showing: 21 - 40 of 51 results

  2. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...homology-directed repair (HDR) to correct double-strand breaks (DSBs). DSB repair by HDR facilitates in-frame... or HA). Tag-specific PCR genotyping can tell if HDR completely failed. If C-terminus tagging potentially...damage (indels) to the promoter region of the non-HDR allele. For N-terminal tagging, specifically searching...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...through exploiting the homology-directed repair (HDR) pathway which occurs in cells at low frequencies... 25-75%, while the success of precise change via HDR limited to 0-5%. This blog post covers a brief review...genome editing in rice and uses base editors and HDR methods for rice crop improvement in the laboratory...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...improvement over Cas9-mediated HDR for the loci tested, where average HDR-mediated editing frequency was... editing efficiency of homology-directed repair (HDR), the pathway for resolving DNA double-strand breaks.... Compared to non-homologous end joining (NHEJ), HDR occurs at a relatively low frequency, especially ...breaking the DNA backbone, sidestepping the problem of HDR vs. NHEJ entirely. The first two classes of base ...

  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...homology-directed repair Homology-directed repair (HDR) stimulated by double strand breaks has been widely...cleavage is relatively high while the efficiency of HDR is relatively low, meaning that most Cas9-induced...most products to be indels while the efficiency of HDR is typically less than 10%. In contrast, the original...desired edits to indel byproducts than Cas9-initiated HDR. Prime editing tools Prime editing is precise and...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...the host cell’s inherent homology directed repair (HDR) process following the double strand break CRISPR...PITCh system plasmids provides an alternative to HDR as the double-strand break repair pathway, relying...

  7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ... you want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or...genome repair by non-homologous end joining (NHEJ) or HDR. EGFP validation of sgRNAs: Can evaluate component...

  9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...types, gene editing using homology directed repair (HDR), and generating large genomic deletions. What are...et al. 2016). For CRISPR experiments that involve HDR, electroporation can be combined with cell-type specific...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... with other large insert techniques like HITI or HDR, which require specific repair pathways to work, ...has higher purity of insertion than both HITI and HDR. PASTE is also compatible with both AAV and adenovirus...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...can efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to ...possible to your desired insertion site to maximize HDR efficiency. In this case, we’re fortunate that there...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...genome via the homology directed repair pathway (HDR), be it a single nucleotide change, insertion of ...mutations by NHEJ and generating small mutations with HDR, when it comes to larger scale genome editing, such...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...the basic science behind homology directed repair (HDR) and how it’s used in CRISPR genome editing.   2....

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations in ATP1A1 can render cells resistant...editing rates as high as 83% with NHEJ and 40-50% with HDR. Coselection efficiency is so high that some applications...

  19. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find the plasmids at...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...editing doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing...
Showing: 21 - 40 of 51 results