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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2 results for: HDR

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  1. Plan Your Experiment

    Type
    Guide
    ...information, read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve ...experimentally verified Cut Nick Homology Directed Repair (HDR) Generate specific genomic edits, like point mutations... Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency...efficiency can be low, but generally higher than HDR pegRNA optimization can be challenging Prime Edit...non-functional form of your protein expressed in your system. HDR : Select a cut site as close as possible to the location...appropriate left and right homology arms. See Addgene's HDR blog post for more details. In some cases, there ...different owing to the error-prone nature of NHEJ . In HDR , base editing , or prime editing experiments, most...

  2. CRISPR Guide

    Type
    Guide
    ...Modifications Using Homology Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits ...but high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template... Both NHEJ and HDR have their uses, but we’ll focus on NHEJ first and come back to HDR . NHEJ is a naturally...that modulate HDR or NHEJ components, like miCas9 , Cas9-DN1S , or others, can increase HDR efficiency. ...base. These small precision edits can be done via HDR, base editing, and prime editing, and are often used...mutation that does not change the amino acid sequence. HDR efficiency is generally low (< 10% of modified alleles...Synchronizing cells or modulating the cell cycle can help as HDR takes place during the S and G2 phases. Chemically...
Showing: 1 - 2 of 2 results