We narrowed to 373 results for: HDR

1. pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

   Plasmid

   #221835

   Purpose

   Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   2xFLAG with dSERT homology arms
   

   Use

   CRISPR

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HDR-HaloPOLQ

   Plasmid

   #211637

   Purpose

   halo donor

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   Puro-LoxHaloTag
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PE7_AAVS1_knockin_HDR_donor

   Plasmid

   #223001

   Purpose

   HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PEmax_AAVS1_knockin_HDR_donor

   Plasmid

   #223000

   Purpose

   HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PEmax
   

   Use

   Hdr donor

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLHDR01_mCherry_tCD19

   Plasmid

   #123661

   Purpose

   Construction of 1st donor for scarless genome editing

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Type

   Empty backbone

   Use

   Mouse Targeting; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLHDR02_EGFP_tCD8

   Plasmid

   #124061

   Purpose

   Construction of 1st donor for scarless genome editing

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Inserts

   EGFP
   tCD8
   

   Use

   Mouse Targeting; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Human UbC promoter modified

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLHDR_R4

   Plasmid

   #124211

   Purpose

   gRNA/Cas9 expression for 2nd editing using pSLHDR04

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDCX_mEmerald_CRISPR_HDR

   Plasmid

   #239331

   Purpose

   HDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locus

   Depositor

   Torsten Wittmann

   Article

   Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

   Insert

   mEmerald and DCX homology arms
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   None

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. makeTCR_HDR_Hs.TRAC_Backbone

    Plasmid

    #233944

    Purpose

    backbone for makeTCR assembly

    Depositor

    Michael Platten

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    knock-in to endogenous TRAC

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gRNA_SOX2_HDR

    Plasmid

    #163752

    Purpose

    gRNA expression vector to target the SOX2 stop codon for HDR gene tagging in human cells.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    gRNA_SOX2 (SOX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLHDR_R1

    Plasmid

    #124209

    Purpose

    gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KHDRBS3_KH_pGEX

    Plasmid

    #135133

    Purpose

    Recombinant expression of KHDRBS3 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    KHDRBS3 (KHDRBS3 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19_HDRT_TRAC_CD19-CAR

    Plasmid

    #183473

    Purpose

    Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

    Inserts

    TRAC (TRAC Human)
    CD19-specific CAR transgene insert
    TRAC (TRAC Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAF-HdrB3

    Plasmid

    #184908

    Purpose

    carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cas9-sgRNA-homology arms
    

    Use

    CRISPR; Broad-host-range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLHDR03_mCherry_tCD19_unique guide

    Plasmid

    #124062

    Purpose

    Construction of 1st donor for scarless genome editing with unique guide

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    mCherry
    tCD19
    

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Human UbC modified

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAFi-dHdrA

    Plasmid

    #184907

    Purpose

    carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cm-sgRNA-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEDF-PhdRS

    Plasmid

    #127445

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A mutations on PylRS

    Promoter

    araBAD

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHDR-USR-SpCas9

    Plasmid

    #208829

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHDR-USR-eGFP

    Plasmid

    #208830

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge