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We narrowed to 461 results for: MEA;

Showing: 21 - 40 of 461 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...with new tools to measure their favorite targets and finding new targets to measure! Let’s take a look...D, et al. A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain...class of signaling receptors, but the options for measuring activity of specific RTKs are limited. In a new...different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this sensor relies...Farahani PE, et al. pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living... affinity isn’t always better, especially if it means operating at the outer edge of the sensor’s dynamic...s41467-022-30685-x. Feel the heat with B-gTEMP  Measuring local temperature variations and heat transfer...

  2. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...spectrophotometer measures these absorbances using UV-transparent cuvettes. First you measure the absorbance...in. This is a “blank” and measures the background absorbance. Then you measure the absorbance of the DNA...common ways to quantify DNA. This method involves measuring the absorbance/transmission of light through a...DNA sample.  These absorbance measures give you an idea of the concentration of your DNA prep and whether...as compared to spectrophotometric methods that measure all nucleic acids). These methods are more sensitive...sensitivity so it’s not used much. However, the measurements are made in the visible range and can be read...

  3. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured using the ...an artificial leaf replica means creating a “leaf” with similar permeability of water and nutrients and... when they find genetic variants, what does that mean? Previous research shows that approximately 75% ...protein abundance, possibly due to misfolding. But measuring variants one at a time is time consuming and unscalable...throughput manner. She helped develop a method that measures the effects of sequence variants on abundance ...sensor in sleep-related studies and in assays for measuring human serotonin transporter activity. Unger also...

  4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...method does provide a quantitative measure of autophagy, it only measures bulk autophagy (as opposed to giving... Long-lived protein degradation: This approach measures the degradation rate of radio-labeled long-lived.... Autophagy is then induced and quantified by measuring the amount of radioactivity in the culture supernatant...out of this assay. This is also a quantitative measure of autophagy, but, like the protein degradation...degradation assay above, it’s slow and only measures bulk autophagy. Morphological assays Electron microscopy... localization in the cell. SEP’s pH sensitivity means Rosella will fluoresce green unless it’s in an acidic... L. K., Graham, T. E. (2015). Rapid parallel measurements of macroautophagy and mitophagy in mammalian...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... anti-fungal drugs, because ‘antibiotic’ merely means that the drug prevents the growth of a living organism...used to treat them. Very often, this is what it means to be a ‘superbug’. Superbugs are often very difficult...antibacterial drugs. Resistance can be intrinsic, meaning a basic characteristic that naturally occurs in...is essentially always there, or acquired, which means the bacterium is not naturally resistant but has...through random mutation or horizontal gene transfer, means to defend themselves from these naturally occurring...inhibited. The amount of inhibition around the disk is measured in millimeters, and corresponds to the level of... seeing a promotion for antibiotic-free, farmed meats. We can focus on creating new antibiotic therapies...

  6. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...Viral Preps from Addgene Measuring the pH of our samples: What we did To measure the pH of our samples, ...few experiments to determine if our tubes were permeable to the CO2 released from dry ice, and whether ... is that some of tubes’ o-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in...the color of the liquid solution and pH paper to measure the pH. We compared the dry ice-stored samples ...Not all tubes (or rather, not all O-rings) were permeable to CO2 at low temperatures. We tested two different...tubes after the indicated incubation periods, and measured the pH. We found that tubes subjected to a recovery...

  7. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...give? Ask yourself if the results mean what the authors say they mean. Could there be any other possible...substantiate the facts upon which it builds. This means you have to become familiar with a body of literature...Finding reliable references and sorting out what they mean is no small task. As a scientist, it is useful to...the work, the research that was done and what it means. The introduction should give you just enough information...experiments, what data they collected and what it means. There should be some connection or flow among the...validating work prior to publication, it doesn’t mean that there are no mistakes or misinterpretations...

  8. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...the hybridoma can occur, known as genetic drift, meaning that over time, the antibody you expect it to produce... the advantage of being significantly smaller, meaning they can recognize epitopes that full antibodies...can be used for qualitative and quantitative measurement of protein expression in cell lysates, whole ...cultured cells. ELISA can be used to quantitatively measure the amount of protein present. The reaction takes... (OD) - of the known and experimental wells is measured. To find the exact amount of protein in your sample...subsets of interest from among the mixture and to measure the relative amount of your protein of interest...the cell surface, or the cell membrane can be permeabilized to allow antibodies to bind to intracellular...

  9. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...group and C, technical replicates or repeated measurements will increase the power of the results. In this...experiment. Technical replicates are repeated measurements of the same sample and are included to assess...the process could make it difficult to obtain meaningful results.  Biological replicates are distinct ...with ‌secondary antibody as usual and the signal measured. If a nonspecific signal is detected, the level...to include a loading control. A loading control measures the protein level of a target that is thought ... the same membrane that the target protein was measured on.    Flow cytometry In addition to the controls...controls for antibody-based experiments, it is by no means an exhaustive list and we encourage you to review...

  10. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...Methods for measuring Dopamine In vivo microdialysis The gold standard method of measuring DA and other...technique is performed with a probe surrounded by a semipermeable membrane, which is inserted into the region ...causes the passive diffusion of DA across the semipermeable membrane from the extracellular fluid into the... the method is powerful and offers immediate measurements in a live animal that can be correlated to behavior...powerful new developments in the detection and measurement of neurotransmitters in various species and while...

  11. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...drop onto the smear until the alcohol runs clear and immediately rinse. Flood the smear with the safranin...a species of bacteria, but what does it actually mean? What relevance does it have on the structure of...pretty simple process. First, flood the heat-fixed smear with the crystal violet stain for 1 minute. Rinse...procedural instructions, including timing, preparing the smear, and mixing the stains and solutions used, check...since gram negative and positive bacteria are permeable to different classes of antibiotics. The classification...

  12. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...is a measure of the on-target activity of the Cas9 nuclease. This can be determined by measuring the indel...Specificity is the measure of off-target activity (left) while sensitivity is the measure of on-target activity...sensitivity. Site accessibility Site accessibility is a measure of which genomic sequences can be targeted by the...frequency at a target site. A specificity ratio can be measured by calculating the on-target activity divided ...

  13. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...trouble if you don’t qualify what kind of “plate” you mean. Without context, it’s difficult to know what kind...official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS tracking system....collaborators will not have the context you do, which means you’ll need to provide that context yourself. Simple...corporate counsel, used the word “service,” did he mean it the same way as Bryan, our customer support manager... meeting is using the same words with the same meanings consistently, meetings will be shorter and more...productive. Higher Productivity. Taking active measures in improving cross team communication has helped...

  14. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration...ddPCR- and qPCR-based titering assays measure physical titers, meaning the concentration of viral particles...Lock et al., 2010). It is recommended that you measure your infectious titer, since it will have more ...

  15. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...your isotype.  Antibody concentration may also be measured through a protein stain, such as Coomassie blue...Though more time-consuming, this assay will also measure the purity of your prep. In this method, antibodies...standard. The density of the antibody bands can be measured against the protein standard and the concentration... beside the heavy and light chains can also be measured. Contaminating bands lower the purity of your ...CN, Vajdos F, Fee L, Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of...monoclonal antibodies with experimental values as measured by the Edelhoch method. Int J Biol Macromol. 2015...

  16. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...that the results can vary by a factor of 2. This means that if you set up two identical assays with the...ddPCR) does not require a standard or a reference, meaning saving on reagents (and time)! AAV titering using...reader extracts the droplets from the plate and measures the fluorescence amplitude of each one. Droplets...outputs an image of the fluorescence amplitude measured for each droplet in each well (Figure 2). A clean...detecting fluorescence so that it is possible to measure the concentration of multiple targets simultaneously...detection of microbes. Some applications include measuring viable probiotics and detecting circulating pathogens...

  17. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...our Transferable Skills series, this post is not meant to be a comprehensive guide to Conflict Resolution...be willing to face conflict head-on. That doesn’t mean you have to like conflict. Very few people do, and...all costs or actively runs away from it. That can mean a willingness to make yourself temporarily uncomfortable... of conflict resolution. Having empathy does not mean you like everyone you meet. Most people can’t maintain... of positivity past the age of five. But it does mean that even if you find someone annoying or disagreeable...party might get exactly what they want, but that means the other party will feel punished or invalidated...

  18. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...binding sites per unit.  There are several ways to measure stickiness. In vitro, ultracentrifugation or gel...Reticulum (OSER) assay (Costantini et al., 2012) measures homodimerization of FPs targeted to the endoplasmid...assessing oligomerization in cells. In addition to measuring stickiness, several researchers have also collected...,” but I find this very confusing because it can mean that the fluorescent protein is at the C-terminus..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3..."A New generation of FRET sensors for robust measurement of Gαi1, Gαi2 and Gαi3 activation kinetics in...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...primary antibodies as needed. However, more antibodies mean more optimization and likely higher background noise.... A wet transfer is the most efficient method, meaning that it will transfer the most protein from your...light-sensitive. They also have a poor dynamic range, meaning that if your blot contains one protein in the femtogram...sensitive but has a limited dynamic range, which means it may take multiple exposures for a blot with varying...you can perform relative quantification, which measures protein amounts and compares the amount of one...use total protein loading. Total protein loading measures overall protein expression and does not rely on...protein expression. Total protein expression can be measured using a protein stain like Coomassie Blue or Ponceau...

  20. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...validate, validate, validate. This doesn’t necessarily mean that you yourself have to perform extensive validation...because an antibody can bind to its target, does not mean that is all it will bind to - particularly when ...performs as expected in one application does not mean that it will perform as expected in another. You...like all of science, validation experiments don’t mean anything unless you share the results with others...consider sharing your validation attempts by other means, such as through antibody databases like Antibodypedia...antibody doesn’t work in your application doesn’t mean it’s a bad antibody but more that it’s misunderstood...
Showing: 21 - 40 of 461 results