We narrowed to 55 results for: MLS

...translates to only about 16 hiPSC colonies per 100 ml of starting blood sample, in the best-case scenario...16 hiPSC colonies can now be obtained from just 1 ml of peripheral blood, a huge improvement from the ...

...extracted from small bacterial cultures (i.e. 2-3 ml) with or without commercially available kits and ...

...primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric...

...filter paper and saturate each with lysis solution (~3 ml each), pouring off any excess. When your protein ...in log phase) in blocking solution (around 108 CFU/mL is a good place to start) and add your membranes ...

...of detection for arg1 expressing ECN is 109 cells/ml, which is within the range of  the ~1011 commensal...

... (concentration, given as viral genome copies/mL (GC/mL)), and total injection volume. You’ll often hear...

...can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of injection. How long post-injection...

...Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). Both rabbit (EMD, AB3596) and mouse (NEB,...

...paper. AAV can be safely concentrated to ~1x1014 vg/ml.. As evident in our repository, this application ...

...antibodies were diluted to 1 µg/mL except for 14558, which was used at 0.35 µg/mL (1/100). * = monoclonal antibody...

...) and grow them in a small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract...

...’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant is only...

...only yield 150 μl of material as opposed to the 15 ml collected from an unconcentrated prep. With this...

...https://doi.org/10.1038/srep31730 Tsuruta LR, dos ML, Moro AM (2018) Display Technologies for the Selection...

...Providenti MA, O'Brien JM, Ewing RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements...

... with half the volume typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture...

...necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer plasmid cultures and then screen...

...cassette, and illustrates how the volume of the 1 ml outgrowth spread on each transformation plate can...

...’s Developmental Studies Hybridoma Bank (DSHB). 1 ml of antibody-containing hybridoma supernatant is only...

...sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically to...