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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 524 results for: PROC

Showing: 21 - 40 of 524 results

  3. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...neurons in the adult dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):E1152-61...foundational later on when we studied biological processes, like how our stem cells are housed in specific...G-deleted RABV can divide and replicate (since these processes are mediated by other viral proteins), but the...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7....

  5. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. USA 112(10) (2015): 3002-7. PubMed...Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression...Aptamer-based assay for prion diseases diagnostic”. Procedia Environmental Sciences. Volume 12, Part B, 2012...

  6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...Recruitment of CRISPR-Cas systems by Tn7-like transposons. Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi....genomic target site bound by QCascade (Fig. 1B). The process also creates a 5-bp target-site duplication (TSD...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...sensitize and kill antibiotic-resistant bacteria.” Proc Natl Acad Sci U S A. 9;112(23):7267-72. (2015). ...Most antibiotics target one of three essential processes: cell wall synthesis, DNA replication, or protein...protein synthesis. Because they target such generic processes, antibiotics don’t just affect the bacteria causing...increased specificity - rather than targeting a process essential to most bacteria, custom CRISPR antimicrobials...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Biol. 2015. PubMed PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312...Science. 2013. PubMed PMID: 24136966 Nyerges, et al. Proc Natl Acad Sci USA. 2016. PubMed PMID: 26884157 ...generating protein chains or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909...relatively well known, particularly in bacteria, the process of cloning, combining, and testing these different...different parts can be quite arduous. The cloning process alone involves much literature searching, DNA synthesis... Boston University have greatly simplified the process of generating multiple E. coli gene expression ...signaling pathways that control many cellular processes such as differentiation, proliferation, and apoptosis...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ....1016/j.chembiol.2012.02.006. 2 Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; 112(1):112-7. doi:... the genome of your favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7....precision in a native context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8...creating a single-molecule reporter of intracellular processes. Multimerization via the SunTag system also shows...conditions, and could initiate specified cellular processes or be observed at a later time via DNA sequencing...Dimer (oLID) could be used to modulate biological processes, but it did not show large changes in binding ...from the laboratory of Ian Frew simplifies the process of creating complex polycistronic lentiviruses....

  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...nanostructures coordinate gene silencing in mature plants. Proc Natl Acad Sci USA 116:7543–7548 . https://doi.org... which contains Vir genes necessary for T-DNA processing and insertion into the plant genome. B) The Ti...

  15. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... higher multiple circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406-13. doi...result of multiple plasmid copies combining in a process known as plasmid multimerization. Plasmid Multimerization...our plasmid samples during our quality control process. Since MiSeq involves sequence assembly from short...plasmid DNA by capillary gel electrophoresis. BioProcess Int. 2006 Sep;4(8):38-40. Summers D. Timing, ... burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci USA 108, 15414–15419. PubMed PMID:...Further, the connections between regions are often reciprocal, forming a neuronal loop which is activated and...

  17. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...output from the first process to the second process. For example, our bbduk process created trimmed FASTQ...pipeline, we wrote a separate Nextflow process. In Nextflow, a process defines which command or script will...specifying the output of an initial process as the input to a subsequent process, we linked each step in our ...pipeline steps into processes and then connect the steps using the output from one process as input to the... Nextflow process.  Next, we specified the input and output channels for the initial process and tested...was clear: to build one process at a time and connect each subsequent process to the existing ones. Once... our first process was working as expected, we moved forward with creating the next process and linking...

  18. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...material transfer agreement (MTA) process. Even though this process is essential to sharing plasmids around...requests and signatures via an online process, though the process has changed a lot over the last twenty...can make our process easier for others.  If you’ve still got questions about our MTA process, you can check...every time they wanted to share materials, and the process was time-consuming and lengthy. So the NIH called...called a meeting in 1990 to discuss the process, which eventually led to the creation of a standardized ...ensure that we can facilitate a fast and agile MTA process. This is important to keep up with the number of...averaged facilitating 89,477 MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually...

  19. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...overview of the QC process at Addgene. All plasmids go through this same initial QC process using an Illumina...script can then continue in the auto-align process. This process is typically reserved for deposits that ...the highest quality and accuracy during the QC process and therefore put in a lot of effort to stay up-to-date...sequencing technologies that Addgene uses in the QC process, when these technologies are used, and how Addgene...first widely used DNA sequencing technology. The process relies on “sequencing-by-synthesis,” where each...by synthesis, but modifies the original Sanger procedure to improve throughput by increasing the number...plasmid components. For more information on this process, see our blog post on NGS plasmid quality control...

  20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...you, while the process has become much more refined and user friendly, to own the process entirely remains...quality data! Addgene's new in-house NGS process To start, the process is only made possible by the use of ... we are able to take greater ownership of this process with in-house sequencing! In addition to sequencing...of our high-throughput DNA isolation process that yields high-quality isolated DNA samples of sufficient...sufficient quantity for sequencing. This process is completed in a plate format yielding anywhere from two to... of 96 samples per week. This is where our new process will begin. Partnering with seqWell, we are using.... From start to finish the library preparation process only takes about one day, even for six plates (...
Showing: 21 - 40 of 524 results