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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 44 results for: PSI-P

Showing: 21 - 40 of 44 results

  1. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ..., Public Library of Science (PLoS), 18 Feb. 2011, p. e16765. Crossref, https://doi.org/10.1371/journal.pone...5, Public Library of Science (PLoS), 30 May 2018, p. e0197185. Crossref, https://doi.org/10.1371/journal.pone...Communications, vol. 2, no. 2, Elsevier BV, Mar. 2021, p. 100135. Crossref, https://doi.org/10.1016/j.xplc.2020.100135...Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The Plant Journal, vol. 106, no. 1,...

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric...Hurtrel, B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or...

  3. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature...with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet ...schematic at the bottom of this post for a basic synopsis of the differences amongst the Brainbow systems...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka, P., Gerfen....L., Kieran, D., Redmond, D.E.Jr., and Aebischer, P. (2010). Efficient transduction of non-human primate... like lentivirus, but instead is contained by a capsid which is comprised of the viral proteins VP1, VP2...genetically engineered to have differences in their capsid proteins. For a brief overview see Addgene’s AAV...

  5. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ....3389/fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (...conjunction with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate ...containing the AAV2 ITRs can be cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019...2019), and different combinations of ITR and capsid serotypes vary in their gene transfer efficiency. ... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced...including commonly used model organisms like Arabidopsis and Medicago truncatula and several crop species...

  7. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci...chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon of useful...

  8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’...very high, ubiquitous expression, while the human synapsin 1 promoter is neuronal specific (Haery et al.,...

  9. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...Thank you to our guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular ... appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources...materials science-based. Light is delivered to your opsin through a small piece of fiber optic cable implanted...stimulation guarantees that the majority of the opsins will receive sufficient quantities of photons to... otherwise optical stimulation will be erratic, opsin activation/inactivation inconsistent, and behavioral...stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex...laser must be controlled. As discussed above, each opsin variant has slightly different activation/inactivation...

  10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, ...focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

  11. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview of the Application of Viruses to Biotechnology...serotypes (12 naturally occurring and more synthetic capsids are routinely being developed) to choose from, ...

  12. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ... element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for normalization...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2, and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...

  13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...Concepcion CP, Bonetti C, Vidigal JA, Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura...other vectors (Lukashev et al., 2016). The protein capsid encapsulating the genome is majorly composed of...Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for monitoring...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...

  15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam A, Windsor I, Travers M, Chen Y, Garcia N,...receptor for SARS-CoV. The lab also found that cathepsins play a role in SARS-CoV infection and deposited...deposited plasmids for the expression of cathepsin S, L, and B. This became a great starting point for SARS-CoV...

  16. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... Therapy returns to centre stage. Nature Vol 526, p.351. PubMed PMID: 26469046. 6. The Forever Fix: Gene...choroideremia, X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing...further improve the technology: engineering of new capsid variants, designing expression cassettes to regulate...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ... 2304918. PubMed Central PMCID: PMC53514 Rowe, W. P., Hartley, J. W., Lander, M. R., Pugh, W. E., & Teich...transcriptase are packaged (Wu et al., 1997) inside of the capsid . This is useful for viral reproduction, and for...for reverse transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully...

  18. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...(is that pun worn out yet?) your research. Derek P. Simon received his PhD in Cellular and Molecular ...drsimonsaysscience.org or follow him on Twitter @derekpsimonphd.    References 1. Stuber, Garret D., et al....derived from direct comparative analysis of microbial opsins." Nature methods 9.2 (2012): 159-172.5. Goshen ...Addgene A Primer on Optogenetics: Introduction and Opsin Delivery The Materials Science of Optogenetics Experiments...

  19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...PMID: 31082625. Ernst, Oliver P., et al. "Microbial and animal rhodopsins: structures, functions, and molecular...Microbial rhodopsins are encoded by opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins...al., 2017). How to get an opsin into the brain In order to express opsins within the brain, researchers... a strong promoter upstream of an opsin gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene...induces first opsin inversion, and then lox sites excision, therefore locking the opsin into the correct... 50 years ago with the discovery of microbial rhodopsins, light-sensitive proteins that absorb photons...light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? Neuronal stimulations...

  20. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...Repurposing retrotransposons for DNA editing By Emily P. Bentley For RNA-guided genome editing, think beyond... systemic PHP.eB capsid, to express either the optogenetic activator channelrhodopsin-2 or fluorescent...
Showing: 21 - 40 of 44 results