We narrowed to 72 results for: SAB

1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...structural information may guide design, it is advisable to generate multiple constructs with different...close to native expression levels. However, it is advisable to first express the fusion protein from a plasmid-based...

2. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

   Type

   Blog Post

   Published

   Oct. 8, 2020, 1:15 p.m.

   ...pipettes for multiple cell lines rather than using disposable ones or reusing gloves. The serum is also a source... ones that are tightly packed with seals. Use disposable pipettes to avoid cross-contamination. Make sure...

3. 2018 #DeckTheLab Contest Winners

   Type

   Blog Post

   Published

   Jan. 15, 2019, 2:19 p.m.

   ...happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk @ShebaAJ...

4. Addgene's Website Update

   Type

   Blog Post

   Published

   April 8, 2024, 2:15 p.m.

   ...little bit faster to skim through. Users with disabilities will find Addgene’s website easier to navigate...

5. Bringing Sustainable Practices to the Lab: Innovations

   Type

   Blog Post

   Published

   March 7, 2019, 2:39 p.m.

   ...waste that needs to be supported at all levels. Disposables are an accepted part of lab culture, but with...

6. Seeing Red: Simple GFP Photoconversion

   Type

   Blog Post

   Published

   Aug. 4, 2015, 2:30 p.m.

   ...FP blog posts.     References Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. Green to ...

7. 28 Hot Plasmid Technologies from 2015

   Type

   Blog Post

   Published

   Dec. 23, 2015, 3:30 p.m.

   ...used to construct 20kb long inserts. The easily reusable MXS-building blocks are particularly effective... Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over...

8. Plasmids 101: Gene, enhancer, and promoter trapping

   Type

   Blog Post

   Published

   May 2, 2023, 1 p.m.

   ...). Is it true that this many genes are truly dispensable or are the current identification methods missing...

9. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   Published

   June 2, 2020, 1:15 p.m.

   ...monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin transcriptional...

10. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    Published

    April 25, 2023, 1:15 p.m.

    ..., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., &...

11. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ... contributes to behavior or pathology, and by disabling neurons you can figure out what they are necessary...

12. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type

    Blog Post

    Published

    Nov. 27, 2018, 2 p.m.

    ...stroke and the third leading cause of death and disability worldwide. An ischemic stroke occurs when arteries...

13. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ... proteins (FPs) have become one of the most indispensable tools in modern biological research.  Any microscopist...

14. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...pRPE. scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd generation lentiviral vector that...

15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type

    Blog Post

    Published

    Sept. 26, 2024, 1:15 p.m.

    ... and incomplete PCR methods yield a minority of usable product with two 5′ overhangs, so these approaches...

16. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...below, you’ll likely be able to create a small, usable panel.  First, fluorophores vary in brightness....

17. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over...

18. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ... and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic screens in human cells...

19. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ... CTD is the second cause of male intellectual disability after Fragile X. There is no cure for CTD, which...

20. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ...fluorophores perform in ‘real experiments’, it is advisable to perform time-lapse imaging of cells producing...