We narrowed to 3 results for: SAB

1. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...purity of plasmid DNA purified without a kit it is advisable to perform a phenol/chloroform extraction of the...contaminants from the plasmid DNA. It is also advisable to add RNAse to the supernatant after step 6 to...

2. Ligation Independent Cloning

   Type

   Protocol

   ...volume with no additional water (<5 μl). It is advisable to set up multiple reactions by holding the vector...

3. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ...very clean DNA, so using a little more enzyme is advisable. Reactions are often performed with 0.2-0.5 µL...