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We narrowed to 72 results for: SAB

Showing: 1 - 20 of 72 results

  1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...they identified mWasabi, a bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter...mNeonGreen. This fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that...relevant changes in pH. Find mTFP1 plasmids here! mWasabi: a bright green fluorescent protein The team that... that excite in the violet range. For example, mWasabi can be used with Sapphire (excitation maximum 399...significantly excite at 400 nm while EGFP does. Find mWasabi plasmids here! One of the most notable things about...localized to their expected locations. An analysis of mWasabi gave similar results. We hope this blog post helped...have plasmids containing mNeonGreen, mTFP1, or mWasabi that you would like to share with the scientific...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...sequences or target sites. Integration of most transposable elements (TEs) results in the duplication of...the site of insertion.  Types of transposons Transposable elements are split into two classes based on...for much of the rest of this article. Class I Transposable elements (TEs): Retrotransposons  Class I TEs...Sleeping beauty Sleeping Beauty is a synthetic transposable element developed from inactivated Tc1/mariner...Sternberg lab developed the INTEGRATE (Insertion of Transposable Elements by Guide RNA-Assisted Targeting) system...

  3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise 73,171 guide RNAs (gRNAs...genes and it includes more guides per gene. The Sabatini/Lander library uses sequential infection first...systematic genetic screens in mammalian cells,” David Sabatini, a senior author of one of the Science papers,... Using the CRISPR/Cas9 System. Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2013 Dec 12. (PubMed)....

  4. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways... with “cloning guru” Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with...attached a DNA barcode to each to make the library usable for pooled screens. Applications of the cancer ...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...dependence. MDV1A and MDV1B were generated by the Sabeti lab using the DELIVER (Tabebordbar et al., 2021...systemic capsids were developed and deposited by the Sabeti lab for use in cynomolgus macaques. PAL1A, PAL1B... M., Ye, S., Solomon, I. H., Tabebordbar, M., & Sabeti, P. C. (2023). Systemic administration of novel...A., Horwitz, N., Beggs, A. H., Wagers, A. J., & Sabeti, P. C. (2021). Directed evolution of a family of.... P., Smith, J., Ibrahim, L. A., Mastro, K. J., Sabri, E., Huang, S., Favuzzi, E., … Dimidschstein, J....

  6. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The Baker Lab focuses on designing...approach. Congratulations to David Baker, Demis Hassabis, and John Jumper! References Kuhlman, B., Dantas...

  8. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...

  9. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... instance, we have a plastic container problem…reusable containers pile up by the sink after lunch as ...thinking about conserving resources in the labs. Disposable plastic materials are fairly inexpensive in this...

  11. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression ...filtered, and titered. A major obstacle in obtaining usable lentivirus is producing a high enough concentration...

  12. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...lentivirus, many organizations require or recommend disposable gowns over your lab coats and double gloving,... of without risking exposure. You can request disposable gowns from your lab or health and safety department...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... PMCID: PMC2593125. 4. Saunders A, Johnson CA, Sabatini BL. 2012. Novel recombinant adeno-associated viruses...these Addgene depositors: Ed Boyden Bernardo Sabatini Wenbiao Chen Liquin Luo Massimo Scanziani Scott...

  14. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...characterized by intellectual and developmental disability, jerky movements, and spontaneous laughter or...by low muscle tone, short stature, cognitive disabilities, problem behaviors, and a chronic feeling of...

  15. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...bottles directly, rather decant the solutions into disposable sterile tubes first. Not only does this make ...and morphology, rendering their continued use inadvisable for experiments requiring reproducible results...

  16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...capability was named INTEGRATE (Insertion of Transposable Elements by Guide RNA-Assisted Targeting) by...sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase complex with the mini-transposon...

  17. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...James McFarland, Jeremie Kalfon, Javad Noorbakhsh, Isabella Boyle, and Joshua Dempster from The Cancer Data...Aaron Fultineer, Jeremie Kalfon, Javad Noorbakhsh, Isabella Boyle, Joshua Dempster, Lia Petronio, Katherine...

  18. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic acid detection...Yozwiak NL, MacInnis BL, Bosch I, Gehrke L, Zhang F, Sabeti PC (2018) Field-deployable viral diagnostics using...

  19. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...party library updates. Third party libraries are reusable software components that can be incorporated into...common name. While we do our best to write clean, reusable code when working on a feature, sometimes we have...

  20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...number. MUCICAT system contains 4 plasmids: pDonor, pTnsABC, pQCascade and pCutamp.  pDonor carries a customizable...form of RE-cargo-LE (RE, right end; LE, left end) pTnsABC expresses the transposases TnsA, TnsB, and TnsC...
Showing: 1 - 20 of 72 results