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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 151 results for: SPEN

Showing: 21 - 40 of 151 results

  1. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and ...Cambridge and Bristol. If you’re a scientist who spends many hours at a microscope, you too can become ...

  2. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...scientists accomplish their goals. Many nonprofits must spend a lot of resources continuously raising money from...self-sustaining. With this unique model, we are able to spend resources on maintaining quality and starting new...

  3. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ... plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...

  4. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ... into your interests and will determine how you spend a good chuck of your mental energy and time. The...At Addgene, we've heard of labs where new hires spend the first 3 months reading and writing a proposal...

  5. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...though derivatives have been adapted for growth in suspension. Of the many derivatives of HEK-293, the most...systems, such as yeast. HEKs can also grow in suspension, which allows for large scalability, and can ...

  6. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...letters after your name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking...entrepreneur at 22. Start with your network You've spent your life making connections and finding mentors...

  7. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ... I was having outside of the “ivory tower.” I’d spend the afternoon lovingly tending to the stem cells...cells I was using in an experiment, then spend the evening listen to some bloke at the pub telling me that...

  9. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...Initially, he spent the majority of his time in the lab.  As time went on and he was spending more time in...

  10. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ... makers to keep things legible. It is faster to spend more prep time on an experiment than it is to rerun...when I was tired, stressed, or rushing. I would spend the time quietly running through the experiment ...

  11. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... the Scientific Support team, much of my day is spent helping to resolve problems, but even so, I wasn...framework to the myriad challenges we encounter. As you spend time in a lab learning techniques and taking on ...

  12. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C ...products on the market to choose from) on your resuspended RNA sample will help ensure DNA contamination...

  13. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... years as a senior scientist at Addgene. Having spent that long in customer service, I've daydreamed about...utility in the least amount of time. Though I now spend far less time answering help emails and phone calls...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...ultracentrifugation of the viral stock, followed by resuspension of the collected particles in a smaller volume...white pellet. The pellet can then be rinsed and resuspended in cold sterile PBS.  Alternatively, to concentrate...

  15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...motivation, but there very few jobs available. So, we spent a lot of time talking about what we could do to ... CSU in 2014. Outside of the lab, Keira enjoys spending time with her husband and two Jack Russell Terriers...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...You’ve spent days and weeks thinking of an amazing project. You’ve written your protocols, designed your...generate templates for the cloning process. Time spent actually doing the experiments are also generally...

  17. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... wear comfortable shoes, as you will likely be spending a few hours on your feet. Pack water and snacks...you ever gone up to a poster where the presenter spends the entire time talking to the poster, as opposed...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ..., homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After finding no cross-reactivity...scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et al., 2020. ...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

  20. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...day, you can save the leftover bacteria-water suspension and use them to start cultures of your positive...up PCR reactions: The remaining bacteria-water suspension will serve as the template for your PCR reaction...
Showing: 21 - 40 of 151 results