Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 6 results for: SPEN

Showing: 1 - 6 of 6 results

  1. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...extraction solution per well and resuspend. Continue to step 7.3 for suspension cells. For adherent cells, ...CRISPR Cloning Anneal and phosphorylate oligos. Resuspend oligos at a concentration of 100 μM in ddH 2 O...detail for murine erythroleukemia (MEL) cells, a suspension cell line. The culture medium in all steps consists...strategies for each cell type. While MEL cells are suspension cells, instructions for adherent cells have ...Ensure there are 2 x 10 6 cells per CRISPR pair. Resuspend 2 x 10 6 cells in 100 μl of electroporation solution...validation (see step 6 ). NOTE: This protocol is for suspension cells. Adherent cells can either grow as individual... gDNA from parental and bulk sorted cells by resuspending parental and bulk cell pellets in 50 μl of DNA...

  2. Zebrafish Plasmid Collection

    Type
    Collection
    ...and non-coding RNAs in zebrafish - Tatjana Sauka-Spengler Lab Lineage Tracing Cell lineage tracing in multicellular...drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific...

  4. Bacterial Expression Systems

    Type
    Collection
    ...helping to conserve cellular resources otherwise spent on producing high amounts of protein. Whether you're...

  5. Antibody Guide

    Type
    Collection
    ...the growth medium and can either be used while suspended in the original media or isolated and placed in...

  6. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids ...
Showing: 1 - 6 of 6 results