Addgene

We narrowed to 798 results for: SPEN

Showing: 1 - 20 of 798 results

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Lenti-sgSpen#1/Cre

Plasmid

#193239

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Spen gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSpen#1 (Spen Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSpen#2/Cre

Plasmid

#193240

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Spen gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSpen#2 (Spen Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_His-CBD-SpEndoH

Plasmid

#218196

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

Depositor

Florence Pojer

Insert

EndoH

Use

Tags

tufA-His-CBD

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 45-313

Promoter

T7

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMCSG17-NleL

Plasmid

#66716

Purpose

Expresses NleL (170-782) in E. coli

Depositor

David Komander

Article

Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

Insert

NleL (NEWENTRY )

Use

Tags

GST-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2

Plasmid

#47891

Purpose

Expresses myc tagged Miro2

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro2 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FRBl2-E

Plasmid

#15289

Depositor

David Spencer

Article

Li et al Gene Ther. 2002 Feb . 9(4):233-44.

Insert

FRAP-rapamycin binding domain (MTOR Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

res. 2025-2113, T2098L

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSH1/Sn-E-Fv'Fvls-E

Plasmid

#15274

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36) (FKBP1A Human)

Use

Tags

HA epitope

Expression

Mammalian

Mutation

FKBP12 F36V

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1

Plasmid

#47888

Purpose

Expresses myc tagged Miro1

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH1/Mf-del-Akt-E

Plasmid

#15288

Depositor

David Spencer

Article

Park et al Nat Biotechnol. 2006 Dec . 24(12):1581-9

Insert

delPH-Akt (Akt1 Mouse)

Use

Tags

HA epitope and Myristoylation-targeting domain Fy…

Expression

Mammalian

Mutation

Truncated PH (residues 1-99)

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSH1/M-Fv-Casp3-E

Plasmid

#15271

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36)-Caspase3 (CASP3 Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12(V36) F36->V

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSH1/M-Fv'-Fvls-E

Plasmid

#15273

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36) (FKBP1A Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12 F36-->V 1st FKBP uses "wobbled&qu…

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV-F-del Casp9.IRES.GFP

Plasmid

#15567

Depositor

David Spencer

Article

Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

Insert

FKBP12(V36)-p30Caspase9 (FKBP1A Human)

Use

Retroviral

Tags

FKBP12 and HA epitope

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 T18N

Plasmid

#47890

Purpose

Expresses myc tagged Miro1 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 T18N (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro1 P13V

Plasmid

#47889

Purpose

Expresses myc tagged Miro1 P13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 P13V (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

P13V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 T18N

Plasmid

#47897

Purpose

Expresses myc tagged Miro2 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 T18N (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 A13V

Plasmid

#47896

Purpose

Expresses myc tagged Miro2 A13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 A13V (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

A13V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 E208K/E328K

Plasmid

#47900

Purpose

Expresses myc tagged Miro2 V208K/E328K mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 E208K/E328K (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

E208K/E328K, abolishes calcium binding

Promoter

CMV

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pSH1/S-Fvls-p30Casp9-E

Plasmid

#15272

Depositor

David Spencer

Article

Fan et al Hum Gene Ther. 1999 Sep 20. 10(14):2273-

Insert

FKBP12(V36)-p30Caspase9 (CASP9 Human)

Use

Tags

HA epitope and Myristoylation-targeting domain c-…

Expression

Mammalian

Mutation

FKBP12 F36 to VTruncated Caspase9 (135-416; lacki…

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-Miro2 R425V

Plasmid

#47898

Purpose

Expresses myc tagged Miro2 R425V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 R425V (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

R425V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 S430N

Plasmid

#47899

Purpose

Expresses myc tagged Miro2 S430N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 S430N (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S430N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only