Addgene

We narrowed to 617 results for: SPEN

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Lenti-sgSpen#1/Cre

Plasmid

#193239

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Spen gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSpen#1 (Spen Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSpen#2/Cre

Plasmid

#193240

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Spen gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgSpen#2 (Spen Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_His-CBD-SpEndoH

Plasmid

#218196

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

Depositor

Florence Pojer

Insert

EndoH

Use

Tags

tufA-His-CBD

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 45-313

Promoter

T7

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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DHB-mVenus

Plasmid

#136461

Purpose

Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

Depositor

Tobias Meyer, Sabrina Spencer

Article

Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

Insert

DNA Helicase B (HELB Human)

Use

Lentiviral

Tags

mVenus

Expression

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SV40-iBlue-pA

Plasmid

#200234

Purpose

Backbone for cloning iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJH124

Plasmid

#102951

Purpose

Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

Depositor

Peter Espenshade

Article

Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

Insert

APEX2-NES

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMCSG17-NleL

Plasmid

#66716

Purpose

Expresses NleL (170-782) in E. coli

Depositor

David Komander

Article

Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

Insert

NleL (NEWENTRY )

Use

Tags

GST-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
U6-TM2Emp-6T_iBlue

Plasmid

#200235

Purpose

Backbone for cloning short RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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Landing_Pad_8xCTS-ECFP-pA

Plasmid

#200242

Purpose

Backbone for cloning 8xCTSs (8xCRISPR-target sites)

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA5_8xCTS-ECFP-pA

Plasmid

#200247

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_8xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3_8xCTS-ECFP-pA

Plasmid

#200245

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_8xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA2_8xCTS-ECFP-pA

Plasmid

#200244

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 2

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA2_8xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA1 _8xCTS-ECFP-pA

Plasmid

#200243

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_8xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA1 _1xCTS-ECFP-pA

Plasmid

#200237

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 1

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA1_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA5 _1xCTS-ECFP-pA

Plasmid

#200241

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA3 _1xCTS-ECFP-pA

Plasmid

#200239

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA4 _8xCTS-ECFP-pA

Plasmid

#200246

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_8xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA4 _1xCTS-ECFP-pA

Plasmid

#200240

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 4

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA4_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA2 _1xCTS-ECFP-pA

Plasmid

#200238

Purpose

Mammalian transfections; 1xCTS-ECFP reporter construct 2

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA2_1xCTS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge