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  1. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...but you’ll only spend half of each day on it. The other half of each day you can spend accomplishing small...’d be too late to meet up, and I’d just end up spending more time in the lab. Obviously I didn’t have ...didn’t realize it until it was too late. I didn’t spend enough time thinking about the possibilities and... moderately useful tasks at the expense of time spent on the long and highly useful tasks. The quick accomplishments...complete the entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The...had general goals for my experiments, but didn’t spend enough time thinking about when or how long it would...
  2. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...budget were proposed. I spent a lot of time asking why. As scientists we spend years being taught to communicate...the solutions they promote help motivate you to spend a little time advocating for science and getting... New Yorker, so it’s not easy to convince me to spend a Saturday in the tourist-trap nightmare that is...disconnected from the entire scientific process.  I've spent a lot of time debating how to fix this.  As scientists...W - Erin Golden Hi, my name is Erin and I have spent the last 15 years training as an academic scientist...
  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...arm (safe). Mice with higher IL-1 beta production spent more time in the open arm and were less risk averse... dark (protected) area. When measuring the time spent in a particular part of the apparatus, it turned...turned out that the IL-1 beta overexpressing mice spent more time in the risky bright place, driven by their...behavioral despair, and the more time the animal spends in the passive mode, the stronger is the depressive...swim test as seen by increasing the ratio of time spent actively swimming versus passive floating. In the...
  4. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ... at Addgene as an Outreach Scientist. I used to spend my days (and often my nights :D) engineering E. ... Pamela Silver’s Lab (plasmid page here). Now I spend much less time wrangling bacteria and instead help...in my position as an Outreach Scientist where I spend a large amount of my time meeting with and talking...on a plane back to Boston from Kentucky where I spent a couple of days talking to researchers about their...
  5. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...A scientist-in-training will spend 10 or more years with a small number of formal advisors learning how...determining whether an advisor will be a good mentor: Spend time with the potential advisor and don’t be shy... in the lab, if possible. Attend a lab meeting. Spend some time hanging out with members of the lab. It...sign if the PI does not welcome you to visit and spend time with everyone. Additionally, if you are looking...
  6. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...can be a major decision. A science trainee will spend 4-7 years working for one person and with a group...gives a 1 hour talk about his or her interests, and spends one-on-one time with the current members to share...solid foundation on which to thrive during time spent as a graduate student or postdoc. Although the chosen...completely happy at work all of the time, the goal is to spend as much time in the "zone" as you can. You know ...
  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...experimental limitations and considerations? I recently spent two months in the lab of Antonello Bonci, Director...The technique is non-trivial; you don’t want to spend months doing surgeries and making fiber optic cables... method of identifying bregma but it is best to spend the time practicing your injections until they are...accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic frame ...
  8. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...University, you are expected to spend at least one semester (the majority spend two to three) working in your...of my six month employment. In the beginning, I spent a lot of time shadowing members of my team to learn...
  9. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ... and reaching out if any milestones are missed. Spend time in person Presenting the results from...circumstances permit, think about having collaborators spend time with each other in person. One possibility ...possibility is to do a lab exchange, where researchers spent time in each other’s labs. This is an opportunity for...
  10. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...Addgene, I had spent most of my junior year stressed out applying for summer programs. I spent one day at ... before high school senior year would have been spent within a lovable community that has allowed me to...
  11. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...to make a plasmid? Or how much time you have to spend cloning before you can start your experiment? What...the most valuable component in science – the time spent by the scientists designing and performing the experiments...but we can assume it’s at least equal to the $155 spent on reagents. Five ways to decrease plasmid production...
  12. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...this method, beads are incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet...Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293-EBNA1 Cells. Methods Mol Biol. 2018;1850...on magnetic beads from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510...
  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...the mix. Thus, our founders and early employees spent this remaining time by elaborately pranking each...play hard.” Part of the reason we sincerely like spending time together is that when it’s time for fun, ...more manageable when you can enjoy the people you spend 40+ hours with each week. In conclusion - get out...
  14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...future work. She graciously took me for coffee and spent an hour with me talking about the tools I could ...rapidly. The knowledge the Singletons gained from spending time with a mentor, as well as having a cohort...participants regarding the activities and time mentors spend with Singletons in their bins. We also sought expertise...
  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings...system An electronics system  Cells are first resuspended in a pressurized buffer called sheath fluid and...instrument clogs, ensure that samples are single cell suspensions. For cells that tend to aggregate, pass the sample...
  16. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...that scientists have amazing stories to share. I spend a lot of time telling scientists about Addgene’s...how to deposit and request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations...
  17. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ... can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges are...
  18. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...biorender.com   Both gradients can be informative, so spend some time thinking about which type of gradient ... your blot’s data, the more time you’ll want to spend optimizing it. Blots for quantification generally...antibodies or validate them yourself, so you don’t spend time troubleshooting an assay that’s fine with antibodies...
  19. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...validated gRNAs can save you the time that would be spent making and testing entirely new gRNA designs. You...demonstrated to work in your system, it is worth spending time sequencing the genomic target to determine...
  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...GFP were published in the 1990s, biologists have spent a lot of time making things glow. Chances are you...spontaneous conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White...
Showing: 1 - 20 of 151 results