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Showing: 21 - 37 of 37 results

  1. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Additionally, alternative Cas enzymes exist, such as Cpf1/Cas12a, which recognizes the PAM sequences of TTTV. While... sequence  Additional notes SpCas9 NGG - Cas12a TTTV Great for AT-rich regions Cas9 EQR NGAG...

  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a may promote...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ... liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers yet published...Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat Commun 10: .https...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    .... elegans. In 2017, the Korswagen lab developed Cas12a (Cpf1) for editing in C. elegans (Ebbing et al....motif required by the Acidaminococcus sp. BV3L6 Cas12a (AsCpf1) eases this constraint and allows editing...

  5. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...cancer research. The Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library...

  9. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and...

  14. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...opt for a completely different Cas effector, like Cas12a (Cpf1). Even more creative options involve generating...
Showing: 21 - 37 of 37 results