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We narrowed to 283 results for: cki

Showing: 21 - 40 of 283 results

  1. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand is also important for ...blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies is whether or ... are viable for translation to human patients. Picking receptors The receptor you choose is largely dependent...

  2. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...Despite these grim statistics, new strategies for attacking cancer are yielding promising results in the laboratory...and balances: protecting against cancerous cells Luckily, there are several safety mechanisms in place to...universally-dividing behavior of cancer cells, attacking the cellular machinery necessary for division....in the field of cancer research has focused on picking apart the complex cellular pathways involved in...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...consider a dual-sgRNA system. This system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic...deleting the catalytic residue of a protein or knocking out a very small peptide. In these cases, you ...sites was sufficient to repress transcription by blocking its initiation. In mammalian cells, robust repression...interest. dCas9-based repressors are useful when knocking out a particular gene isn’t feasible. For example...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...the intensity of fluorescence signal and enables tracking of single molecules within living cells without...roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell cycle. Good...used with your own reporters to decipher their trafficking routes. This collection is now available at Addgene...traditional cloning strategies may seem preposterous. Luckily, the Pierre Neveu laboratory designed a new iterative...and an endoplasmic reticulum export motif and trafficking sequence for improved targeting to the plasma...studying membrane dynamics, for monitoring cargo trafficking, and for discovering organelle contact sites..... This appears to contribute to more efficient blocking of read-through transcription for pXPG. The other...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...self-excising cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression...2013), or rescue of mutant phenotypes (unc-119) (Dickinson et al., 2013). These approaches allowed effective...e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion...for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred for studying...Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast Saccharomyces cerevisiae...

  7. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...immunohistochemistry images that are crisp, bright, and lacking in non-specific staining can be a challenge in ... mixing your primary and secondary antibodies, blocking excess secondary with serum from the same species...0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

  8. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...throw a therapeutic monkeywrench into the virus’ hijacking of human cells. Even better, more than half the...the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at a time Since...headache receiving constant email requests and tracking their fulfillment manually in a shared spreadsheet...

  9. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited...-CamKII-ArchT-GFP (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators...

  10. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...bind to amine groups present in some conjugates, blocking the conjugation reaction. Many commercial antibodies...for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out, however...in your machine before you make your purchase. Picking a single fluorophore for an experiment is easy,...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...components: the error prone PolI3M that’s fused to a nicking Cas9 (nCas9). Similar to mutagenesis with error...error-prone polymerase (PolI) that is fused to a nicking Cas9. A guide directs Cas9 to a site of interest...changes promote nCas9’s dissociation from DNA after nicking and yielded an enhance nCas9 (enCas9) EvolvR with...

  12. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...definitely recommend checking out GCE4All’s website and resources, and then checking out all of the plasmids...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...

  13. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...genome randomly, while others use attP sequence docking sites so that you can control exactly in which ...at different loci. Fortunately, there are many docking sites available for integration. 2. How soon would...would know beforehand on which chromosome your docking site is located). This amounts to a small difference...

  14. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... it’s monumentally difficult without software. Tracking lab procedures also gives you access to hard data...repeated. We also now have a good interface to begin tracking analytics data for lab procedures. With this data...are distinct from their contents. You should be tracking both, you should easily be able to move contents...

  15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...localization in cells, or even single-particle tracking of the bound complex. There are so many FPs available...TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells... can even be bright enough for single-molecule tracking in live cells.  Controls  When designing experiments...

  17. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...Miyatsuka et al.) Distinguish between models for trafficking to the lysosome by fusing Medium-FT to LAMP-2A...structure and a particular biological event (trafficking to a subcellular location, start of gene expression...change color from blue to red report on cellular trafficking." Nature chemical biology 5.2 (2009): 118-126...

  18. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...programs is almost as daunting as the program itself. Picking a mentor comes with many challenges, and the flood... to potential mentors?” “Where do I start with picking universities?” and “How does COVID-19 impact all...into what matters to you. It can also be nerve wracking to ask questions in front of other prospective...

  19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...in “crispr” and see what comes up. Before even clicking the ‘Search’ button a new content-aware suggestion... your lab. Instead of starting a new search or clicking through to a different part of the website, you...functionally (e.g. Base Editing) and by target organism. Clicking through to RNA Editing brings us to a page with...

  20. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...each sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate ... download the entire analysis as a ZIP file by clicking the Download Analysis Data button on the bottom...
Showing: 21 - 40 of 283 results