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We narrowed to 166 results for: crispr c plasmids

Showing: 21 - 40 of 166 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... 79628 (C) Cv (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635 NS3 C 79637...79637 NS2B C 79636 NS4A N 79640 NS4B C 79639 NS5 C 79632 (N) and 79631 (C) PrM N and C   Detecting...Find Lourido CRISPR Plasmids. Sidik and Huet, et al. Cell. 2016. PubMed PMID: 27594426 CRISPR-X: dCas9-...Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ... you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over...

  2. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ....org Read the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...Guide RNAs Visit our CRISPR topic page Learn the basics of CRISPR Download the CRISPR 101 eBook Resources...j.cell.2021.09.018 Choi, J., Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza,...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally..., J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A.,...variants in most cell types. But prime editing, a CRISPR technique developed in David Liu’s lab in 2019,...considerably more precision and flexibility to the CRISPR editing world. The approach, originally published...

  3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...about CRISPR Pooled Library Experiments Additional resources on the Addgene website Find CRISPR Plasmids...on your CRISPR background with our CRISPR Guide Find Software Tools for gRNA Design    ...updated in 2022 by Susanna Stroik.  The advent of CRISPR/Cas9 has made it easier than ever to make precise...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity... be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits Is the location...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...additional RNA-binding transcriptional activator. Plasmids for activating target genes in a variety of species...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources page...    The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To find...New auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service ...Tonia Rex's lab.  vfCRISPR, or very fast CRISPR, is a light-inducible CRISPR system developed by Taekjip...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...cleavage can induce DSBs within minutes. New CRISPR plasmids for Borrelia burgdorferi from Christine Jacobs-Wagner...

  5. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...Browse gRNA Design Tools Read Our CRISPR Guide Pages Find CRISPR Plasmids  ...120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016). ...not generated from a plasmid. If you, for example, prefer to deliver your CRISPR components as RNPs (ribonucleoproteins...Design Your gRNA Use the CRISPR Software Matchmaker Browse Alll of Our CRISPR Posts Additional Resources...For example, entering ‘C. elegans’, or ‘elegans’ will filter the table to show C. elegans gRNA sequences.... Many of the gRNAs contained within these plasmids have been used successfully in peer reviewed articles...articles. If you’re targeting your favorite gene with CRISPR, using one of these validated gRNAs can save you...

  6. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ...

  7. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ...Editing CRISPR Cheat Sheat Resources from Addgene.org CRISPR Guide CRISPR: Protocol for Genome Deletions in...systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME Knocks out a pair... Addgene blog CRISPR101: Validating Your Genome Edit How to Design Your gRNA for CRISPR Genome Editing...time-consuming, as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells...and in combination with different genes. Having CRISPR systems for assessing genes in defined contexts...  The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow...

  8. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Addgene.org Catch up on your CRISPR background with our CRISPR Guide Browse CRISPR plasmids for base editing Find...Addgene Blog CRISPR 101: Homology-Directed Repair CRISPR 101: Cytosine Transversion Editors CRISPR 101: Any...based on Cas9 off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing...fourth-generation base editors, BE4, reduce the undesired C→G or C→A conversions that can happen with BE3s. These...Compared to BE3, BE4 offers a 2.3 fold decrease in C→G and C→A products as well as a 2.3 fold decrease in indel... Hong, M., Zhang, D., Luan, C., Zhang, S., Wu, H., Gao, H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z... W., Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt...

  9. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our CRISPR Guide Check out ...Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, or C base editors...Resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors CRISPR 101: Cytosine Transversion...transversion editors: CGBEs, or C → G Base Editors. CGBEs first convert a cytosine (C) to uracil (U), just like... a more specific A → C base editor (ACBE), they inserted the deaminase into the C-terminal domain of Cas9...pyrimidines (C ↔ T).   Base transversion Base change from purine (A or G) to pyrimidine (C or T) or vice-versa...type of base editor invented. CGBE C → G Base Editor Converts C → G by fusing a uracil DNA glycosylase...

  10. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources ...acssynbio.0c00192   The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New...system, showing the workflow for inducible CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy...New ready-to-use viral preps New toolkit for CRISPR-based genome editing in organoids By Ashley Waldron...

  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...Tools for an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids 101: The Promoter Region...Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov, R., Fort, A., Gebhard, C., Goldowitz, D., Guhl,...Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998). Potent and specific genetic interference...

  12. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...transversions C → TG → A A → GT → C C → G                   C → AG → C                   G → T A → C                   ... Yang, C., Dong, X., Ma, Z., Li, B., Bi, C., & Zhang, X. (2022). Pioneer Factor Improves CRISPR-Based ...Rosser, S. J., Bi, C., & Zhang, X. (2021). Glycosylase base editors enable C-to-A and C-to-G base changes...blog CRISPR 101: Cytosine and Adenine Base Editors Read about four base editor reporters CRISPR 101: ...Editing CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids...editing Get a broad overview with our CRISPR Guide Check out our CRISPR 101 ebook ...Liu, D. R. (2021). Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis...

  13. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ....org Read our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein is 1,241..., IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans... also led to the discovery of IgnaviCas9, a new CRISPR enzyme from a sample from Mound Spring in Lower...throughput and rigorous assembly. There, they found a CRISPR array in the resulting Ignavibacteriae phylum genome...temperatures for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol... Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another thermostable...

  14. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...almost 18,000 CRISPR plasmids in our repository, and we have shared almost 300,000 CRISPR plasmids and 12,000...all these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing...Diverse CRISPR Plasmids by Addgene. The CRISPR Journal, 6(6), 493–501. https://doi.org/10.1089/crispr.2023.0059...all of biology…CRISPR.  Since the discovery of CRISPR, the labs that developed CRISPR tools were committed...who have deposited CRISPR plasmids (Figure 1C), and the cumulative number of CRISPR libraries Addgene ... Figure 1: Addgene CRISPR distribution and deposits. (A) Cumulative CRISPR plasmids shipped by year. (...laboratories that have deposited CRISPR plasmids by year. (D) Cumulative CRISPR pooled libraries shipped by year....

  15. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...Addgene blog: Browse CRISPR articles from our CRISPR topic overview Find other CRISPR methods for bacteria...Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids from Addgene ...integration using CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems....beneficial for plasmid clearance. It is more convenient to update the spacer(20nt) on pEcgRNA plasmid as only.... pCutamp cures the plasmids by targeting the ampR promoter in all three plasmids. pCutamp is then removed... 13 plasmids with 14 rounds of transformation in 30 days. Figure 2: (A) MUCICAT plasmids for ...Yang lab, we’re continually optimizing the use of CRISPR editing systems in bacteria. Our pCas/pTargetF ...

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use Cas9...M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. Efficient CRISPR/Cas9-assisted ...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...delivery Epitope tagging of mammalian genes using CRISPR depends on successful delivery of three reagents...using custom synthetic two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by function... with 40–60% G/C content are most likely to be successful, although sequences with G/C content outside...of the 3′ extension of the pegRNA should not be C. A C base is speculated to base pair with G81 of the..., J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A.,...editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer binding site ...Prime Editing: Adding Precision and Flexibility to CRISPR Editing PRIDICT: Predicting Efficiencies of Prime...function Browse our entire plasmid collection ...

  18. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are...Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of the CRISPR." The newest genome...purposes that we had to create a CRISPR page to keep up with all the new plasmids. So far, we have distributed...

  19. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources More resources on...unexpectedly affect nearby genes (Figure 1A-C). Find CRISPR plasmids at Addgene! Knock-in: Introducing tags... DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are powerful tools. CRISPRi uses a... for us, CRISPR is an incredibly versatile system that can be tweaked to target ncRNA. CRISPR/Cas9 techniques...Bester 2021.    Alternative CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely...If the CRISPR/Cas9 system is not effective for your experiments, there are plenty of other CRISPR-based....996067 Basak J, Nithin C. Targeting Non-Coding RNAs in Plants with the CRISPR-Cas Technology is a Challenge...

  20. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here.        New toolkit for N or C terminal tagging...Kanemaki lab has created a new toolkit of plasmids for the N- or C- terminal tagging of proteins with mAID...targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely...2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method to tag endogenous proteins in...and other tags (Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein...
Showing: 21 - 40 of 166 results