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We narrowed to 78 results for: fluorophore

Showing: 21 - 40 of 78 results

  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...you image the first target, you inactivate the fluorophores in the sample, either by chemical treatment ...and that each secondary has a different color fluorophore that you can image separately on your microscope...labeling sequentially with one, inactivating the fluorophores, then labeling with the other, you can still...using primary antibodies that are conjugated to fluorophores. However, the primary-secondary workflow (a....fluorescently-conjugated streptavidin to get more fluorophores per antibody, or conjugate with an enzymatic...

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...strategies. The green structure represents a fluorophore or other useful ligand, gray structure is the...non-natural amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to...years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability...Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores...better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell, L. P., McDougall, M. G.,...

  4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...their technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered..., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the...clearing methods and can be labeled by synthetic fluorophores, which are more stable than fluorescent proteins...see systems that use newer, more photostable fluorophores and that can be combined with new imaging techniques...

  5. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

  6. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...

  7. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

  8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited...useful in multicolor labeling particularly with fluorophores that excite in the violet range. For example...

  12. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...

  13. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...light source, a cell expressing the associated fluorophore or stained with the associated fluorochrome, ...fluorescence. A cell expressing high levels of the fluorophore or fluorochrome-stained protein will emit a stronger...expression, measured using antibody conjugated fluorophores Fluorescent antibodies get around the challenges...

  16. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...been introduced to GFP and new iterations of fluorophores are constantly being engineered. Table 1 lists... 1: The specific mutations comprising common fluorophores Fluorescent Protein Mutations Relative to...

  17. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...However, the excitation light excites all of the fluorophores as it passes through the specimen, which often...obstacles (e.g. air bubbles or high concentration of fluorophores) cast shadows along the illumination path. The...

  19. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... bind specifically and with high affinity to fluorophores. Examples are the FlAsH tag, the His tag and...enzyme to covalently label the peptide with a fluorophore. Both systems are a hybrid between a genetically...
Showing: 21 - 40 of 78 results