We narrowed to 34 results for: gc

1. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ...position; (5) avoid overhangs with either 0% or 100% GC.) In fact, the NEB® team’s data showed that high-...

2. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ... Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based...

3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   Published

   July 2, 2020, 12:40 p.m.

   ...Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In Situ Capture...

4. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...References 1. Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible...

5. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...plasmids just won’t amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or...

6. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Oct. 14, 2015, 2:30 p.m.

   ...that of Cas9 (3’-NGG) in both genomic location and GC-content. After sequencing and searching for cellular...

7. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...mismatches more distal to the PAM, and 2) guides with high GC content have greater off-target activity. Short gRNAs...

8. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...after a ‘G’ base because CBE is inefficient in a ‘GC’ sequence context. If this cannot be avoided, select...

9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ...vector that you want to use is very large, has a high GC content, contains a lot of repeats--any of which ...

10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    Published

    July 28, 2016, 2:30 p.m.

    ...nt length, ~60 oC annealing temperature, and ~50% GC content) so that individual parts and/or strings ...

11. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ... (concentration, given as viral genome copies/mL (GC/mL)), and total injection volume. You’ll often hear...

12. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...provided in the original reference library, as well as GC%, reverse compliment sequences, and more. Output_...

13. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type

    Blog Post

    Published

    Nov. 3, 2015, 3:30 p.m.

    ... guide RNA (sgRNA) design, and the abnormally low GC content of the Plasmodium falciparum genome. To put...

14. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...than 3 degrees between the two Tms)  Aim for 50-60% GC content, preferably weighted towards the ends of ...