We narrowed to 35 results for: gc

...plasmids just won’t amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or...

...References 1. Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible...

...efficiency such as the length of the RTT overhang, the GC content of the primer binding site, and many more...

...Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In Situ Capture...

...position; (5) avoid overhangs with either 0% or 100% GC.) In fact, the NEB® team’s data showed that high-...

... Park J, Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based...

...that of Cas9 (3’-NGG) in both genomic location and GC-content. After sequencing and searching for cellular...

...mismatches more distal to the PAM, and 2) guides with high GC content have greater off-target activity. Short gRNAs...

...after a ‘G’ base because CBE is inefficient in a ‘GC’ sequence context. If this cannot be avoided, select...

...vector that you want to use is very large, has a high GC content, contains a lot of repeats--any of which ...

...nt length, ~60 oC annealing temperature, and ~50% GC content) so that individual parts and/or strings ...

...provided in the original reference library, as well as GC%, reverse compliment sequences, and more. Output_...

... (concentration, given as viral genome copies/mL (GC/mL)), and total injection volume. You’ll often hear...

... guide RNA (sgRNA) design, and the abnormally low GC content of the Plasmodium falciparum genome. To put...

...than 3 degrees between the two Tms)  Aim for 50-60% GC content, preferably weighted towards the ends of ...