We narrowed to 256 results for: idua
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TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m....Letters of recommendation wisdom Reach out to 2-3 individuals in your professional circle to vouch for the ...for internship applications. Thus these noble individuals are inundated with requests. The easier you make...the wrong approach. It is very likely you know individuals in your circle that will also benefit from these...
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New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....exciting new tool that allows researchers to individually label millions of mammalian cells through lentiviral...ClonTracer library allows a researcher to label individual members of a starting population of cells with...multiplicity of infection (MOI) such that each individual cell receives only one barcode. The abundance... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....make their own decisions about genetics, both as individual consumers of technologies and as societal participants...three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines, and...is to liaise with different organizations and individuals, to identify ways of collaborating, and to organize... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual colonies by (carefully) holding plates up to ...identified from the plate circling- are then individually punched out, gently sucked up into the pipettor...useful to scientists who wish to rapidly isolate individual cell clones with different expression characteristics... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....lines, looking to uncover genetic dependencies of individual tumors, which can lead to new cancer targets ...analysis. Their work revealed that cell lines from individuals of African descent have approximately 20% more...particularly in cancers that are more common in individuals of African descent.Correcting the bias While... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....of the experiment would require access to each individual video. To PLOS’s credit, the organization subsequently...recording are highly variable and usually left to individual researchers. Yet accurate and comprehensible ...appears to leave the scope of data to be shared to individual authors. Minimally, raw but sufficiently annotated... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m....After that, the next gates fully depend on your individual experiment. Practical example: lymphocytes in...strategy, I can, of course, also draw multiple individual gates around the populations of interest (Figure...of a quadrant gate, I am free to use multiple individual gates. Note that in this case, square gates were... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....immunodeficiency syndrome (AIDS) in untreated individuals. Symptoms of AIDS include rapid weight loss and...opportunistic infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate...CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected with HIV-1. For two HIV... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....red-green). Even better, show each channel in individual panels, then the multicolor overlay; this helps...primary antibody in your experiment). Confirm each individual target is stained correctly Image a set of samples...label only one target in each. Compare these individually-labeled samples with the results of your multiplex-labeled... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....consistently welcoming. This bears repeating. Individuals that work in science policy are a treasure--they...do to creatively connect seemingly disparate individuals and ideas toward solving one problem. It often...school or a postdoc. But some are also open to individuals from mid- to late-career backgrounds. In fact... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m.... the initial stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type ...maintenance involved. Also it was really fun to find individual colonies growing out in the 96-well dish. My ...control GFP gRNA (BRDN0000561167) (80034-LV) were individually used to transduced stable pools of Cas9-A549... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m.... at multiple levels to generate anything from individual transcription units (TUs) used to express your...metabolic pathways. For many MoClo systems, the individual parts are reusable, saving time and money on ...array of DNA parts that can be assembled into individual TUs or very large (up to 20!) multi-TU constructs... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m.... different parts of the brain is mediated via individual neurons. Neurons are composed of a cell body,...and aid in visualization of the morphology of individual neurons, including dendritic and axonal extensions...these neurons fluoresce in different colors. An individual neuron can then be traced throughout the brain... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog PostPublishedNov. 8, 2016, 3:30 p.m.... hopes that these screens will both identify individuals who are infected with HR HPV and therefore at...HPV subtypes with risk and disease severity. Individuals who are shown to be at high risk by these screens... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....out a career path? Many trainees are developing Individual Development Plans (IDP's) through Science Careers... References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork... -
Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m....Vectr has layers, which let you lock and hide individual vectors (shapes defined by 2D points connected...biology, medical specialities, and general items. Individual images as well as collections of images are easy... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....disease progression or onset. These hits can reveal individual genes or sometimes even genetic pathways that...generate patient mutations and then test both individual and combinatorial drugs on for effectiveness ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m.... sentences that I found hard to understand or individual words that are not appropriate for the given ... attention to details and use a checklist for individual sections of the paper (see figures). After finishing... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m.... a survey instrument designed to measure how individuals respond to issues of unsettled science. Understanding...building, each day brushing past and chatting with individuals whose research ranged from wildlife conservation... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....your selection method kills the transgene free individual. For example, with CRISPR-Cas9 gene editing,...experiments can only be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous...