We narrowed to 62 results for: kan

1. 27 Hot Plasmids from 2016

   Type

   Blog Post

   Published

   Dec. 22, 2016, 3:03 p.m.

   ...Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using CRISPR technology...

2. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

   Type

   Blog Post

   Published

   June 8, 2023, 1:15 p.m.

   ...doi.org/10.1038/s41596-021-00644-9 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola...

3. pSiM24: Simplifying Plant Genetic Engineering

   Type

   Blog Post

   Published

   Sept. 29, 2015, 2:30 p.m.

   ...functional in a variety of plant species. The kanamycin antibiotic resistance gene is controlled by a ...

4. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   Published

   May 12, 2015, 1:32 p.m.

   ...Voojis M, Beverloo HB, Al Allak B, van Drunen E, Kanaar R, Berns A, Jonkers J. Proc Natl Acad Sci USA. ...

5. 28 Hot Plasmid Technologies from 2015

   Type

   Blog Post

   Published

   Dec. 23, 2015, 3:30 p.m.

   ...contains a nonfunctional truncated version of the kanamycin resistance gene (missing the first two codons)...pUCXKT will therefore have resistance to both kanamycin and ampicillin (from the pUC19 backbone). *False...

6. Plasmids 101: Antibiotic Resistance Genes

   Type

   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ...Class Mode of Action*   Working Concentration** Kanamycin aminoglycoside Binds 30S ribosomal subunit; ...

7. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   Published

   Oct. 29, 2015, 2:30 p.m.

   ...resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102, with EcoRI and selected...

8. The Importance of High Titer for AAV Transductions

   Type

   Blog Post

   Published

   March 1, 2018, 2:11 p.m.

   ...remeasure your titer after diluting to confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working...

9. Four Factors that Differentiate the Stem Cell Field

   Type

   Blog Post

   Published

   Sept. 28, 2016, 2:35 p.m.

   ...discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal cord injuries...

10. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...resistance cassettes is most commonly employed, with the KanMX being the most prevalent due to its versatility ...

11. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell...

12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...densely populated places like the University of Kansas and the University of Louisville in Kentucky, the...

13. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ...133–138. PubMed PMID: 20010839. 4. Caussinus, E., Kanca, O., and Affolter, M. “Fluorescent fusion protein...

14. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11):e3647. doi...

15. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...Neuroscience Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators...

16. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

17. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin...

18. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...1, 18-28. PubMed PMID: 22428977. 2. Choi, V.W., Asokan, A., Haberman, R.A., & Samulski, R.J. (2007). Production...

19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ... protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for...

20. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...