We narrowed to 59 results for: nin

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Aug. 18, 2015, 2:30 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Feb. 13, 2025, 2:15 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.

Join Addgene for Depositor Week from November 16 to 20, 2020 as we celebrate the role of Addgene depositors in open science!

Nov. 16, 2020, 2:15 p.m.

Addgene lab technician Racheal Komuhendo describes her recent experiences at a workshop at Lab Central and how you can get the most out of a similar workshop.

Feb. 20, 2018, 2:30 p.m.

Use this transfection calculator when packaging your AAV vectors.

Jan. 23, 2018, 2:04 p.m.

Check out how you can use digital PCR to validate your CRISPR genome edits.

March 30, 2017, 2:30 p.m.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

April 12, 2016, 2:30 p.m.

This blog post explores ways to gain leadership experience at professional organizations like the National Postdoctoral Association.

Aug. 6, 2015, 2:30 p.m.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

April 16, 2015, 3:45 p.m.

Summary and thoughts from the post-doc driven 2014 Future of Research Conference in Boston.

Oct. 7, 2014, 3 p.m.

Learn about what's coming for Addgene in 2026

Feb. 5, 2026, 4:15 p.m.

Making the transition from bench to management can be daunting, but these tips can help!

Jan. 21, 2025, 2:15 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

Struggling to get started with designing your first flow panel? This blog walks you through what you need to know and an example panel design to help you get started!

May 28, 2024, 1:45 p.m.

There are many thing to consider when designing a scientific poster: font size, layout, background, and color. Learn more about poster design in this blog post

Oct. 22, 2019, 12:20 p.m.

Designing a cloning experiment? Read this blog post to learn about different positive and negative selection strategies you can take to find the clones you're looking for.

Aug. 22, 2019, 12:43 p.m.

The academic job interview chalk talk is essentially a million dollar sales pitch about your research vision. Learn how to define your research vision, organize your chalk talk, and practice efficiently before your next interview!

March 12, 2019, 1:37 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.