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We narrowed to 262 results for: rigi

Showing: 21 - 40 of 262 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...of Cas13a. The origins of Cas13a: An RNA cleaving CRISPR nuclease Cas13a was originally identified in ... whether the target sequence is present in the original pool of nucleotides.   What are the potential...the applications of CRISPR. Since this post was originally published, we've also seen the new development...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...broad host range. These features are: The plasmid origin of replication (ori) contains structural elements...For example, the pJD4 plasmid harbors distinct origins (ori1, ori2, ori3) and replication initiation proteins...Konieczny, I., & Helinski, D. R. (1998). Replication Origin of the Broad Host Range Plasmid RK2: Positioning...More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids 101: What is a Plasmid?...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...turning transcription OFF. Despite their bacterial origins, Tet systems function well in mammalian cells, ... previous post. In the past 30 years since the original Tet-Off system was described by Gossen and Bujard...inducible manner detailed in our previous post. While originally developed for use in mammalian cells, this system...work best for your experiments! This post was originally written by Mary Gearing in 2018, and updated ...

  4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...other contains the bacterial selection marker and origin of replication. During bacterial culture, only ...plasmid with the antibiotic resistance gene and origin will replicate, and the transgene will be lost....contains the antibiotic resistance gene (AmpR) and origins of replication (ori and f1 ori) which allow it ...

  5. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...put down a basic outline and refer back to the original paper that everyone in your lab has been referencing... down to inadequate information about what the original researchers actually did. Admittedly, the differences...President of Editorial at Cell Press.  She trained originally in cell biology, completing her PhD at the MRC...

  6. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...cell type they are tranfected into – a bacterial origin of replication (ORI) will not allow for plasmid...considered an entirely new cell line from that of the original parental cells. Positive selection in mammalian... 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All Plasmids 101 Articles ...

  7. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ... signals from time to time. False positives in original studies and false negatives in replications are...replication study. An important difference between the original setting and the new one, previously unaccounted...when replications do not come out the same as an original research finding we can be more certain that some...

  8. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

  9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...per cell division and thus, genes closer to the origin of replication will be overrepresented compared...divided. This means that genes located near the origin will be over-represented compared to those further...promoters drove maximal gene expression near genomic origins of replication, the stabilized promoters created...

  10. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ... changes in intracellular Ca2+ levels than the original version containing CRY2 without the extra peptide...conversion into xanthine. The majority of this operon originates from Pseudomonas putida CBB5 and has been tailored...lab and the UT Austin undergraduate iGEM team originally used a guaB knockout E. coli strain to assay ...

  12. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take...we explore… the life and times of the myc tag.  Origins of protein tags For about as long as the disciplines...mouse IgE, goat IgG, (and more!) as well as the original mouse IgG1. And the refinement of this trusty ...

  13. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... cell migration (Sladitschek and Neveu, 2015). Origin and purpose of MXS chaining MXS-chaining was designed...digestion followed by ligation. Upon ligation, the original pattern of the MCS is regenerated at the flanks...approaches in mammalian cell culture systems (their original goal). Their work has demonstrated that the MXS-chaining...

  14. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...to solve your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford...starting point, their ideas scored 37% higher in originality. It may not be easy to optimize virus production...References and Resources References Grant, A. (2017). Originals how non-conformists move the world. Penguin Publishing...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...relating to reproducing the original research can be documented -- in the original context in which it was...import Word documents into RSpace, maintaining the original formatting. In this way data is moved from a static...

  16. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...machinery making an original C-G pair converted to T-A or reverted to the original C-G by base excision... ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair is converted to a G-C pair...

  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...indicates that the cancer has spread from where it originated to another organ in the body. Such misdiagnosis... cells that may have spread beyond the point of origin, and radiation to stop or slow the growth of tumors... before it starts to spread beyond its point of origin. One of the most exciting developments recently...

  18. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...tools and assumptions to reveal findings that the originator was oblivious to, no matter how much time they...productivity”, so this is unlikely. Usually, the originating scientist and colleagues are privileged in viewing...generators. This practice doesn’t appear to harm the originating researchers, even if they are occasionally beaten...

  19. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...DNA template used for repair is identical to the original DNA sequence at the DSB, or it can introduce specific...newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in DSBR, following...

  20. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...through equal opportunities for all regardless of origin. It is therefore essential that internationalization...experience from talented professionals to their original countries to help build, together with the universities...frontiers. Moreover, sharing a culture/country of origin with an international mentor creates a closer and...
Showing: 21 - 40 of 262 results