We narrowed to 135 results for: tTA

...looper moth (Potter et al., 1976). Its target site is TTAA and unlike other transposons, it does not leave ... its preferred target integration site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian...have a specific integration target site, such as TTAA for Sleeping Beauty, they can insert at any number...

... strategies include using engineered ligases to attach a ligand to a peptide tag on your protein of interest...standard confocal microscopy. Image adapted from (Bottanelli et al., 2016) under CC-BY license. Combining...experiments!   References and resources References Bottanelli, F., Kromann, E. B., Allgeyer, E. S., Erdmann...

...2. The Immortal Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African American...

...virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking...

...fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome to Montana...

...lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve ...serve as controls in a variety of experiments, while rtTA can be used to activate expression of a gene under...

...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol...

.... to understand cancer and defeat it we need to attack it from all fronts!”  Here I aim to compile a few...are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis The conference...

...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...bacterium to make a small “bridge” or sex pilus that attaches to the recipient cell drawing it close. Once in...

... protein. These RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they...group of the ribose to protect it from nucleolytic attack. Another stabilizing sugar modification, found ...

...separate them. Most proteins are not likely to remain attached to the DNA throughout the sample processing and...sample may need more or less cross-linking to firmly attach the proteins to the DNA, depending on how tightly...

... used to turn “on” and “off” MEK1 activity by attaching two dimerizing photoswitchable Dronpa (dron= a...at sites homologous to the locations they were attached to in MEK. The photoswitchable versions of MEK1...

...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...reality, multiple primary antibodies can and do attach to a single target protein, so this assumption ...

...genes that help bacteria invade hosts, including attaching to host cells and evading the host immune system...resistance plasmids, which protect the host from attacks by other organisms. To conserve energy, these specialized...

...Despite these grim statistics, new strategies for attacking cancer are yielding promising results in the laboratory...universally-dividing behavior of cancer cells, attacking the cellular machinery necessary for division....

...geothermalis (Dge10TnpB) targets a different TAM (5′-TTAT-3′). The unique TAM requirements expand the number...5: Colony Screening Use Primer 92F (5′-cattacgcaattggacgacaac-3′) and Oligo 2 (specific to your target...

...covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...

...are small plastic containers that can easily be attached with a hose to a nitrogen gas tank with a pressure...encoded by the plasmids in the library will remain attached to the membrane at the same location that their...

... at the terminal 3’ PAM site (Fu et al. 2013; Pattanayak et al. 2013). This approach misses larger mismatches...25595557. PubMed Central PMCID: PMC4308725. 7. Pattanayak, Vikram, et al. "High-throughput profiling of...