...live-cell imaging, the use of dyes that exhibit low toxicity and high photostability is crucial. These properties...dye's ability to enter cells without causing undue toxicity. A prominent example is DAPI, a dye that binds...in live cells can sometimes result in cellular toxicity due to the permeation process. Advanced technologies...of fluorescently tagged nanocellulose for nanotoxicological studies. Environmental Science: Nano, 6(5)...

...transduction) targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate...cells showed up to 70% resistance to diphtheria toxin, indicating a 70% frequency of biallelic mutations... by nature transient, which may lower nuclease toxicity or the potential for off-target effects. Third...

...replication in mammalian cells, for example, and a toxin that kills bacteria may not have any discernable... a cassette that will make cells resistant to a toxin), whereas negative selection would be the picking... a cassette that will make cells sensitive to a toxin). In the table below we focus on positive selection...

...Long-term light exposure can also lead to cellular phototoxicity, and experimental success can be affected by...-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple subcellular...imaging, especially where photobleaching and phototoxicity are a concern, these constructs come in very...

...core of this tool is the endoprotease Botulinum Neurotoxin B (BoNT/B), which cleaves the synaptic protein... to SspB, brings together the split botulinum neurotoxin halves to enable VAMP2 cleavage, and suppresses...photodimerizer system CRY2-CIB1 to regulate botulinum neurotoxin were also created. The depositors demonstrated...

...starting knock-in experiments. Test dTAG molecule toxicity in new cell lines Treat parental cells (or cells...biological assay of choice to ensure that there are no toxicities at the doses used to evaluate degradation of ...have evaluated to date, we observed little to no toxicity with dTAG-13 at doses as high as 20 µM (see Figures...

... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

... cool things about amp is that it's exclusively toxic to dividing cells. The other antibiotics listed ...across systems and species. The cost? Zeocin is genotoxic when not 100% inhibited, meaning it will induce...there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy et al...

...genetic tools to the brain because of its low cytotoxicity, low immunogenicity, and the long-term expression...of FPs can trigger protein aggregation which is toxic for the cells and affects the labeling of small ...fluorescence. Furthermore, many of these FPs are cytotoxic, prone to aggregation and cross-react with anti-GFP...

...signs of toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin...

...antigens found in the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs...tumor site. ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer...facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR has an scFv...

...severing and has very low dark-state activity and toxicity. All of that means this light-powered sword truly... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks interested in ...

...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...

...fluorescence. Since emission of green light is more phototoxic to cells, most scientists working on in vivo ...fluorescence when exposed to blue light, which is less phototoxic to live tissue than the UV light used by other... a reduced potential for photobleaching and phototoxicity that standard FPs might induce though their ...

...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Mülleder M, et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout pooled library Parasites from... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

...these DNA samples must also be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall...

...and auxotrophy) and negative selection (ex: toxin-antitoxin systems and sacB counter-selection) tools used...

...of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov...

...want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind...they contain large inserts or genes that create a toxic product. The E. coli strain Most E. coli strains...

...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...