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We narrowed to 599 results for: REV;

Showing: 191 - 200 of 599 results

  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC -3’; Reverse oligo: 5’- AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC... Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities...

  2. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ...mutations in the PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from... congenital disorders of glycosylation (CDG): a review. J. Inherit. Metab. Dis. 39, 765–780 (2016). PubMed...acetylcholine. Given its unique molecular biology and previous approval by the FDA, GdnHCl could, in principle...

  3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...Editing Technology at Beam Therapeutics, used a previously developed bacterial selection strategy (Gaudelli...more efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017...mitigated with the expression of fetal hemoglobin. Previous studies have shown that one base change in the...

  4. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...field forward to where it is today. Below we will review some of the CRISPR-based editing therapies that...’s own cells that have been modified ex vivo, previously edited healthy donor cells can be delivered immediately...safety is important for allogenic therapies to prevent a graft vs. host response. Off-target effects are...

  5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-051410...transcription start site function would also help to reveal the impacts of a lncRNA on nearby genes and its...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...therefore already have ways to prevent AAV infection. For example, prevalence of neutralizing antibodies ...or potentially curing human diseases that were previously thought to be incurable. Rapid advances in next...

  8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...symposia held across the USA during this time have revealed that young scientists are aware of, and are engaged...Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95). For those advocating...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

  9. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...a table. It adds a formality and distance that prevents interaction with each other and the audience. ...moderator takes control It is the moderator's job to prevent anyone from derailing the show. The moderator must... active at all times keep things moving and to prevent any one panelist or audience member from taking...

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... series from Subach et al.) were derived from previously developed monomeric fluorescent proteins mKO ...dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer.... German. "Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009...
Showing: 191 - 200 of 599 results