...organizers. My overall impression from this conference was that Cancer Researchers and Immunologists...Here I aim to compile a few highlights from the conference which reflect how cancer/immunology researchers...multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting presentation by...our Fluorescent Protein Guide. Throughout the conference aspirin and the use of COX inhibitors was discussed...about our free deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction...

... scientific conference, whether you are attending in person or monitoring that conference via social media...American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly know how I was selected to participate...like Fast Company, opportunities to speak at conferences, and offers for postdoc positions. Check out ...s worth a read since many of the pointers are different from the other links shared in the post. And the...

...tools to further their research. However, tools aren’t very useful if you don’t know how to use them. ... ideas come from social media conversations, conferences, and questions our scientific support staff receives...line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer ...for our blog posts, we usually make figures in BioRender or use appropriately licensed and cited figures... We are constantly reviewing the resources we currently offer to identify gaps and add to them as techniques...

...developers work in a streamlined manner. Now, in my current role at Oxford Nanopore Technologies, I work with... part of my job is getting to talk to so many different people! I communicate with customers, software...Often, product managers will have three or more concurrent projects in varying stages, which can get messy...meetings. Meet with my boss and coworkers, discuss current and future features being built for the analysis...blogger Rachel Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore...

...Linearized Plasmids After PCR). Current site directed mutagenesis methods Current SDM methods rely on in vitro...those to PCR amplify the template plasmid. The parental, nonmutated DNA is digested with methylated DNA-specific...common methods for site directed mutagenesis   References 1. Trehan, Ashutosh, et al. (2016). “REPLACR-... on Addgene.org Browse Our Molecular Biology Reference Pages Choose Your Perfect Plasmid Backbone Find...

...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE...repair are easily re-cleaved while indel products aren’t, so repeated cycles will favor accumulation of ...Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM...2014) Is Non-Homologous End-Joining Really an Inherently Error-Prone Process? PLoS Genet 10:e1004086 ....

...protein expression journey! Resources and References References Nabet et al. The dTAG system for immediate...expression of essential proteins (when knockouts aren’t viable). siRNA or RNAi are small, double stranded...days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 ..., and expression is tunable by titration with different amounts of tetracycline. Tetracycline regulation...

...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’...bispecific, meaning that each scFv can target a different antigen; binding of each can be either simultaneous...convert anyone to downsize their affinity reagents, trendy as that may sound. Validated antibodies are still... a scientist looking for that perfect fit!  References and Resources Additional resources on the Addgene...AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and ...

...dependence on excitation power is important since different fluorescence imaging strategies use widely varying..., TIRF or spinning disk confocal use entirely different excitation power regimes. Therefore, it is unpredictable...the best way to compare the photostability of different fluorescent proteins? Measuring photostability...excitation power. By repeating these measurements for different fluorescent proteins and by comparing how the ...measurements reported in the literature are performed in different ways. We describe a number of issues with some...

...1013 - 1014 vg/kg, a dosage comparable to those currently used in human gene therapy trials. They further...reproducibility as scientists can assemble parts from different labs and experimental systems in their own research...mutants, determined protein expression levels from different promoters, and inhibited gene expression using...C-terminus of Cre (CreC) and the N-terminus of Cre (CreN) are fused to the truncated Phytochrome B (PhyBΔ...eye, muscle and epithelial cells, as well as in different stages of development, ensuring the breadth and...

...Emily P. Bentley in October 2024. References and Resources References Demanèche, S., Bertolla, F., Buret...functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids: microbial defense... also causes outbreaks of food poisoning, the difference is virulence plasmids! This category includes...Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid is among the most...

...whenever you hear it (whether at a scientific conference or a karaoke bar), you probably want to sigh....methyltransferase 1 (DNMT1) to copy methylation marks from parental to daughter strands of DNA. When dCas9 is bound...nearly 100% demethylation without the confounds inherent to dCas9-TET, allowing one to study the causal... CRISPR-based tools for epigenetic editing.   References and resources:  Additional Resources on the Addgene...Addgene.org Read our CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) ...

... can only carry around 4.5 kb. Different AAV serotypes have preferences for specific cell types. For example...end of an experiment series would behave very differently (distracted) from the ones tested at the beginning...Experiment Behavioral scoring systems (experimenter differences in behavior observation); experimenter blinding...viral service for their behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene...

...28032891. This is a different version of Split-BioID. BirA* is fragmented at a different site than in Schopp... This can artificially select against PPIs that aren't stable outside the native cell context. The Split-BioID...of the BirA* fusion matters! Schopp et al saw different levels of fusion protein expression and biotinylation...research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak, J., Kreibich...

...or undetectable FRET, well-characterized FRET reference standards can be used to validate FRET measurements...sophisticated questions about cellular processes. Currently, FRET experiments can probe protein-protein interactions...Borghi 2016) to a selected protein. Overcoming current limitations to permit the use of multiple FRET ... diseases and even assist in drug discovery. References 1. Baird, Geoffrey S., et al. "Circular permutation...biomolecular structure and dynamics in live cells." Current opinion in structural biology 34 (2015): 52-59....

...mammalian plasmid here and edit on! References and Resources References Kwart, D., Paquet, D., Teo, S. et...sequence constraints, or at least has looser or different requirements. Luckily, there are some available...necessarily more flexible than NGG; they’re often just different (e.g. NGAG, NGAN, etc.). Additionally, alternative...al). Additionally, SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver Lab (read...

... to address the unique needs of students from different departments across the university and alterations...track postdocs nationwide. On this level, FoR is currently compiling a database of institutional responses...implementation of new strategies designed to cope with current increases. These two rulings along with progress...Howell -Founder of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University...public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical...

...Several protocols have been written outlining the different steps and materials required for each [1, 2]. ...discussed above, each opsin variant has slightly different activation/inactivation kinetics. For example,...Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne... or follow him on Twitter @derekpsimonphd.    References 1. Gutierrez DV, et al. Optogenetic control of...channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial board, Jacqueline...

...antibodies, context is key.    References and Resources References Evan GI, Lewis GK, Ramsay G, Bishop...cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding to linear sequences... or the C-terminus, Evan, et al. isolated six different monoclonal antibodies (Evan, et al. 1985). Three...-9E10 variable regions and sticking them onto different scaffolds. But over the years, several alternative...

...Find the MagLOV plasmid here!   References and resources References Duret, G., Polali, S., Anderson,...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...be developed from other fluorescent proteins.  Currently, the team is working to identify how MagLOV’s ... of many optogenetic tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a ...